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北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第59巻 第3号 >

脂肪酸分析のための蛍光誘導体化試薬9-アントラセンメタノールの精製

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Title: 脂肪酸分析のための蛍光誘導体化試薬9-アントラセンメタノールの精製
Other Titles: Purification of 9-Anthracenemethanol as a Fluorescent-Labeling Reagent for Microanalysis of Fatty Acids
Authors: 端場, 祐介1 Browse this author
安藤, 靖浩2 Browse this author →KAKEN DB
Authors(alt): Haba, Yusuke1
Ando, Yasuhiro2
Keywords: 9-Anthracenemethanol
Fatty acid analysis
Fish oil
Recrystallization
High-performance liquid chromatography
Issue Date: 8-Dec-2009
Publisher: 北海道大学大学院水産科学研究院
Journal Title: 北海道大学水産科学研究彙報
Journal Title(alt): Bulletin of Fisheries Sciences, Hokkaido University
Volume: 59
Issue: 3
Start Page: 67
End Page: 72
Abstract: A fluorescent-labeling reagent 9-anthracenemethanol was purified by recrystallization for use in microanalysis of natural oil fatty acids. A commercial reagent was dissolved in ethyl acetate at the boiling point, and allowed to stand at room temperature until the crystals precipitated in yellow needles. The product given in the yield of 74% was analyzed by reversed-phase high-performance liquid chromatography. Many contaminant peaks found in the crude material disappeared in the chromatogram, indicating successful purification of the reagent. The purified reagent could be used for fatty acid analysis of 1-5μg of natural triacylglycerols including those of cod liver/mackerel oil and bonito head oil. 2-Butanone was also effective solvent for the purification. With these solvents, commercially available 9-anthracenemethanol can be purified by the simple recrystallization.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/40204
Appears in Collections:北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第59巻 第3号

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