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ウニ病害原因菌 Vibrio sp. の同定のための 16S rRNA 遺伝子を標的にした特異的 PCR の開発

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Title: ウニ病害原因菌 Vibrio sp. の同定のための 16S rRNA 遺伝子を標的にした特異的 PCR の開発
Other Titles: Development of 16S rRNA targeted PCR for the identification of Vibrio spp., the causative bacteria of the discase in cultured sea urchin Stronglylocentrotus intermedius occurring at low water temperatures
Authors: 曽我部, 陽子1 Browse this author
田島, 研一2 Browse this author
田中, 礼士3 Browse this author
澤辺, 智雄4 Browse this author →KAKEN DB
絵面, 良男5 Browse this author
Authors(alt): Sogabe, Yoko1
Tajima, Kenichi2
Tanaka, Reiji3
Sawabe, Tomoo4
Ezura, Yoshio5
Keywords: エゾバフンウニ
低水温期病害
Vibrio spp.
16S rRNA
PCR
Issue Date: 15-Mar-2002
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Journal Title(alt): Bulletin of the Japanese Society of Scientific Fisheries
Volume: 68
Issue: 2
Start Page: 201
End Page: 206
Publisher DOI: 10.2331/suisan.68.201
Abstract: The causative bacteria, Vibrio spp., have been isolated from the diseased sea urchin Strongylocentrotus intermedius at low water temperatures. To develop a specific identification method for the pathogens, species-specific nucleotide sequences in the 16S rRNA of Vibrio spp., were determined. We designed two PCR primers, DA2F and SK1F, based on the nucleotide sequences : DA2F forward primer (nucleotide numbers 456 to 476 in Escherichia coli 16S rRNA) for Date isolates and Sk1F forward primer (nucleotide numbers 455 to 478 in E. coli 16S rRNA) for Shiriuchi and Shikabe isolates. The PCR product with about 1kbp was obtained in all causative bacteria from Date, Shiriuchi, Shikabe Breeding Centers and some of Vibrio reference strains by amplification with DA2F-1540R primer set, however, those from three Breeding Centers differed from the reference strains in the case of growth at 30℃. PCR amplification with SK1F-1540R primer set was able to differentiate the causative bacteria from Date and those from Shiriuchi and Shikabe. These results indicate that PCR amplification with two primers is useful for identification of Vibrio spp., which are the pathogens of cultured sea urchin.
Rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである
© 2002 公益社団法人日本水産学会
© 2002 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/42513
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺 智雄

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