Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR

Fukui, Youhei; Sawabe, Tomoo

doi:10.1264/jsme2.23.172


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Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/42537

Title:	Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR
Authors:	Fukui, Youhei Browse this author
Authors:	Sawabe, Tomoo Browse this author →KAKEN DB
Keywords:	quantitative real-time PCR
	Vibrio harveyi
	rapid detection
Issue Date:	Jun-2008
Publisher:	Japanese Society of Microbial Ecology
Journal Title:	Microbes and Environments
Volume:	23
Issue:	2
Start Page:	172
End Page:	176
Publisher DOI:	10.1264/jsme2.23.172
Abstract:	Vibrio harveyi cause mass mortalities of cultured marine fish. To address the ecology of V. harveyi in aquaculture, intensive monitoring is needed. We first optimized a quantitative real-time PCR method to determine V. harveyi abundance. The designed TaqMan probe and primers based on the 16S rRNA gene were specific at 68℃ of annealing and extension. Furthermore, the method using a chelating resin method was able to enumerate 1.7×10^2 CFU/ml in breeding seawater at an abalone farm. This method represents a good tool for monitoring the ecology of V. harveyi in marine environments within 5 h.
Description:	Short Communication
Rights:	日本微生物生態学会 (Japanese Society of Microbial Ecology)
Rights:	本文データは学協会の許諾に基づきCiNiiから複製したものである
(Relation)isversionof:	http://ci.nii.ac.jp/naid/110006684370
Type:	article
URI:	http://hdl.handle.net/2115/42537
Appears in Collections:	水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺智雄

OAI-PMH ( junii2 , jpcoar_1.0 )

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