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Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR

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タイトル: Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR
著者: Fukui, Youhei 著作を一覧する
Sawabe, Tomoo 著作を一覧する
キーワード: quantitative real-time PCR
Vibrio harveyi
rapid detection
発行日: 2008年 6月
出版者: Japanese Society of Microbial Ecology
誌名: Microbes and Environments
巻: 23
号: 2
開始ページ: 172
終了ページ: 176
出版社 DOI: 10.1264/jsme2.23.172
抄録: Vibrio harveyi cause mass mortalities of cultured marine fish. To address the ecology of V. harveyi in aquaculture, intensive monitoring is needed. We first optimized a quantitative real-time PCR method to determine V. harveyi abundance. The designed TaqMan probe and primers based on the 16S rRNA gene were specific at 68℃ of annealing and extension. Furthermore, the method using a chelating resin method was able to enumerate 1.7×10^2 CFU/ml in breeding seawater at an abalone farm. This method represents a good tool for monitoring the ecology of V. harveyi in marine environments within 5 h.
記述: Short Communication
Rights: 日本微生物生態学会 (Japanese Society of Microbial Ecology)
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 澤辺 智雄


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