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Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR

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Title: Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR
Authors: Fukui, Youhei Browse this author
Sawabe, Tomoo Browse this author →KAKEN DB
Keywords: quantitative real-time PCR
Vibrio harveyi
rapid detection
Issue Date: Jun-2008
Publisher: Japanese Society of Microbial Ecology
Journal Title: Microbes and Environments
Volume: 23
Issue: 2
Start Page: 172
End Page: 176
Publisher DOI: 10.1264/jsme2.23.172
Abstract: Vibrio harveyi cause mass mortalities of cultured marine fish. To address the ecology of V. harveyi in aquaculture, intensive monitoring is needed. We first optimized a quantitative real-time PCR method to determine V. harveyi abundance. The designed TaqMan probe and primers based on the 16S rRNA gene were specific at 68℃ of annealing and extension. Furthermore, the method using a chelating resin method was able to enumerate 1.7×10^2 CFU/ml in breeding seawater at an abalone farm. This method represents a good tool for monitoring the ecology of V. harveyi in marine environments within 5 h.
Description: Short Communication
Rights: 日本微生物生態学会 (Japanese Society of Microbial Ecology)
本文データは学協会の許諾に基づきCiNiiから複製したものである
(Relation)isversionof: http://ci.nii.ac.jp/naid/110006684370
Type: article
URI: http://hdl.handle.net/2115/42537
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤辺 智雄

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