|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species
| Title: | A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species |
| Authors: | Yasui, George Shigeyuki Browse this author | | Arias-Rodriguez, Lenin Browse this author | | Fujimoto, Takafumi Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | cryopreservation | | fish | | inseminating dose | | loach | | Misgurnus | | sperm |
| Issue Date: | Dec-2009 |
| Publisher: | Elsevier |
| Journal Title: | Animal Reproduction Science |
| Volume: | 116 |
| Issue: | 3-4 |
| Start Page: | 335 |
| End Page: | 345 |
| Publisher DOI: | 10.1016/j.anireprosci.2009.02.021 |
| PMID: | 19446413 |
| Abstract: | The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30℃ for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (Dimethyl sulfoxide - DMSO, Dimethylacetamide - DMA, glycerol - Gly, ethylene glycol - EG, and methanol - MeOH at 0, 5, 10 and 15%), external cryoprotectants (Bovine serum albumin 1 and 2%; sucrose 0.5 and 1%; glucose 0.5 and 1%; glycine 0.5 and 1%), activating solutions (distilled water, dechlorinated tap water, 25 mM NaCl and 50 mM NaCl), and hatchability of the eggs when fertilized with fresh or cryopreserved sperm. After the evaluation of these parameters, we optimized the cryopreservation using the following procedure: thawing temperature at 25℃ for 10 s; loach or cyprinid extenders; methanol at 10 or 15% as internal cryoprotectants; glycine 0.5% or bovine serum albumin 1% as external cryoprotectants and 50mM NaCl for sperm activation. Using this procedure, the fertilizability of the post-thawed sperm was 47% in comparison to the fresh sperm, at the minimum inseminating dose (687.65 sptz egg^[-1]mL^[-1]). Based on this protocol, sperm from other loach species Lefua nikkonis, M. mizolepis and Barbatula toni were cryopreserved successfully. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/42542 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 荒井 克俊
|
