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A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species

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タイトル: A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species
著者: Yasui, George Shigeyuki 著作を一覧する
Arias-Rodriguez, Lenin 著作を一覧する
Fujimoto, Takafumi 著作を一覧する
Arai, Katsutoshi 著作を一覧する
キーワード: cryopreservation
fish
inseminating dose
loach
Misgurnus
sperm
発行日: 2009年12月
出版者: Elsevier
誌名: Animal Reproduction Science
巻: 116
号: 3-4
開始ページ: 335
終了ページ: 345
出版社 DOI: 10.1016/j.anireprosci.2009.02.021
抄録: The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30℃ for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (Dimethyl sulfoxide - DMSO, Dimethylacetamide - DMA, glycerol - Gly, ethylene glycol - EG, and methanol - MeOH at 0, 5, 10 and 15%), external cryoprotectants (Bovine serum albumin 1 and 2%; sucrose 0.5 and 1%; glucose 0.5 and 1%; glycine 0.5 and 1%), activating solutions (distilled water, dechlorinated tap water, 25 mM NaCl and 50 mM NaCl), and hatchability of the eggs when fertilized with fresh or cryopreserved sperm. After the evaluation of these parameters, we optimized the cryopreservation using the following procedure: thawing temperature at 25℃ for 10 s; loach or cyprinid extenders; methanol at 10 or 15% as internal cryoprotectants; glycine 0.5% or bovine serum albumin 1% as external cryoprotectants and 50mM NaCl for sperm activation. Using this procedure, the fertilizability of the post-thawed sperm was 47% in comparison to the fresh sperm, at the minimum inseminating dose (687.65 sptz egg^[-1]mL^[-1]). Based on this protocol, sperm from other loach species Lefua nikkonis, M. mizolepis and Barbatula toni were cryopreserved successfully.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/42542
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 荒井 克俊

 

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