HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species

Files in This Item:
arai_ARS116.pdf228.83 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/42542

Title: A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species
Authors: Yasui, George Shigeyuki Browse this author
Arias-Rodriguez, Lenin Browse this author
Fujimoto, Takafumi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: cryopreservation
fish
inseminating dose
loach
Misgurnus
sperm
Issue Date: Dec-2009
Publisher: Elsevier
Journal Title: Animal Reproduction Science
Volume: 116
Issue: 3-4
Start Page: 335
End Page: 345
Publisher DOI: 10.1016/j.anireprosci.2009.02.021
PMID: 19446413
Abstract: The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30℃ for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (Dimethyl sulfoxide - DMSO, Dimethylacetamide - DMA, glycerol - Gly, ethylene glycol - EG, and methanol - MeOH at 0, 5, 10 and 15%), external cryoprotectants (Bovine serum albumin 1 and 2%; sucrose 0.5 and 1%; glucose 0.5 and 1%; glycine 0.5 and 1%), activating solutions (distilled water, dechlorinated tap water, 25 mM NaCl and 50 mM NaCl), and hatchability of the eggs when fertilized with fresh or cryopreserved sperm. After the evaluation of these parameters, we optimized the cryopreservation using the following procedure: thawing temperature at 25℃ for 10 s; loach or cyprinid extenders; methanol at 10 or 15% as internal cryoprotectants; glycine 0.5% or bovine serum albumin 1% as external cryoprotectants and 50mM NaCl for sperm activation. Using this procedure, the fertilizability of the post-thawed sperm was 47% in comparison to the fresh sperm, at the minimum inseminating dose (687.65 sptz egg^[-1]mL^[-1]). Based on this protocol, sperm from other loach species Lefua nikkonis, M. mizolepis and Barbatula toni were cryopreserved successfully.
Type: article (author version)
URI: http://hdl.handle.net/2115/42542
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University