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An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia
| Title: | An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia |
| Authors: | Suzuki, Minoru Browse this author | | Takahashi, Yoshinori Browse this author | | Nakano, Satoru Browse this author | | Abe, Tsuyoshi Browse this author | | Masuda, Michio Browse this author | | Ohnishi, Toshiyuki Browse this author | | Noya, Yoichi Browse this author | | Seki, Koh-ichi Browse this author |
| Keywords: | Laurencia sp. | | Rhodomelaceae | | Brominated metabolites | | Biosynthesis | | 82Br-compounds | | TLC-ARLG method |
| Issue Date: | Jul-2009 |
| Publisher: | Elsevier Ltd. |
| Journal Title: | Phytochemistry |
| Volume: | 70 |
| Issue: | 11-12 |
| Start Page: | 1410 |
| End Page: | 1415 |
| Publisher DOI: | 10.1016/j.phytochem.2009.07.038 |
| PMID: | 19733868 |
| Abstract: | The production of labeled brominated metabolites with radioactive 82Br in Laurencia species was investigated as part of a study of the biosynthesis of halogenated metabolites from species belonging to the red algal genus Laurencia (Rhodomelaceae, Ceramiales). Radiobromide [82Br], thin-layer chromatography (TLC), and TLC-autoradioluminography (ARLG) were used. When cultured in artificial seawater medium (ASP12NTA including Na82Br) under 16:8 h light:dark (LD) illumination cycles for 24 h, each of the strains of Laurencia, Laurencia japonensis Abe et Masuda, Laurencia nipponica Yamada (laurencin-producing race and laureatin-producing race), and Laurencia okamurae Yamada, produced species- (or race-) specific 82Br-containing metabolites. In the case of the laurencin-producing race of L. nipponica, laurencin and deacetyllaurencin were found to be produced in approximately 1:1 ratio, though laurencin is the major metabolite in the wild sample. Furthermore, when cultured in the dark, the production rates of brominated metabolites in Laurencia spp. were found to be diminished. The present study strongly indicates that the use of radiobromine [82Br] in combination with the TLC-ARLG method is an effective approach for investigating the biosynthesis of brominated metabolites in Laurencia. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/42544 |
| Appears in Collections: | アイソトープ総合センター (Central Institute of Isotope Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 関 興一
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