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Induction of permiablity and apoptosis in colon cancer cell line with chitosan

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Title: Induction of permiablity and apoptosis in colon cancer cell line with chitosan
Authors: Hossain, Zakir Browse this author
Takahashi, Koretaro Browse this author →KAKEN DB
Keywords: Chitosan
Caco-2 cells
HT-29 cells
tight junction
Issue Date: 2008
Publisher: 行政院衛生署藥物食品檢驗局
Journal Title: 藥物食品分析
Journal Title(alt): Journal of food and drug analysis
Volume: 16
Issue: 5
Start Page: 1
End Page: 8
Abstract: The effect of chitosan on tight junctions (TJs) permeability on Caco-2 cell monolayer intestinal model was investigated. We have also investigated the effects of low molecular weight water-soluble chitosan (LMWC) on apoptosis in HT-29 cell. The changes in barrier properties of Caco-2 cell monolayers, including transepithelial electrical resistance (TEER) and permeability to lucifer yellow, were assessed in response to chitosan treatment. High molecular weight chitosan (HMWC) was found to cause up to 50% dose-dependent reduction in TEER of Caco-2 cell monolayer without damage to the cell membrane under lower concentration. The effect of HMWC on TJs was confirmed by increased permeability of lucifer yellow when cells were treated with 0.001-0.0001% HMWC for 120 min compared to control cells. Results showed that HMWC did not affect the F-actin of cytoskeleton. LMWC was proven to be an antitumor compound as shown by inducing apoptosis as a function of DNA fragmentation. These results suggest that HMWC is an useful drug delivery agent in paracellular pathway and LMWC has potential value in colon cancer therapy.
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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