HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
人獣共通感染症リサーチセンター  >
雑誌発表論文等  >

Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis

VAC27-52_7339-7345.pdf1.17 MBPDF見る/開く

タイトル: Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis
著者: Dang, Zhisheng 著作を一覧する
Yagi, Kinpei 著作を一覧する
Oku, Yuzaburo 著作を一覧する
Kouguchi, Hirokazu 著作を一覧する
Kajino, Kiichi 著作を一覧する
Watanabe, Junichi 著作を一覧する
Matsumoto, Jun 著作を一覧する
Nakao, Ryo 著作を一覧する
Wakaguri, Hiroyuki 著作を一覧する
Toyoda, Atsushi 著作を一覧する
Sugimoto, Chihiro 著作を一覧する
キーワード: Echinococcus multilocularis
Primary alveolar echinococcosis
発行日: 2009年12月 9日
出版者: Elsevier Ltd.
誌名: Vaccine
巻: 27
号: 52
開始ページ: 7339
終了ページ: 7345
出版社 DOI: 10.1016/j.vaccine.2009.09.045
抄録: Echinococcus multilocularis causes an important zoonotic cestode disease. The metacestocle stage proliferates in the liver of intermediate hosts including human and rodents and forms multiple cysts. Recently, members of a transmembrane protein tetraspanin (TSP) family have been used as vaccines against schistosomosis, or as diagnostic antigens for cysticercosis. In this study, seven tetraspanins of E. multilocularis, designated as TSP1 to TSP7, were evaluated for their protective potential against primary alveolar echinococcosis. The large extracellular loop (LEL) region of these tetraspanins was cloned from a full-length enriched cDNA library of E. multilocularis metacestodes and expressed in Escherichia coli as a fusion protein with thioredoxin. Recombinant TSPs were applied as vaccines against an E. multilocularis primary experimental infection in BALB/c mice. Cyst lesions in the livers of vaccinated and non-vaccinated mice were counted. The cyst lesion reduction rates induced by the seven tetraspanins in vaccinated vis-à-vis non-vaccinated mice were: 87.9%, 65.8%, 85.1%, 66.9%, 73.7%, 72.9% and 37.6%. Vaccination conferred protective rates to mice ranging from 0% (TSP5, 6, 7) to maximally 33% (TSP1, 3). The results indicated that recombinant tetraspanins have varying protective effects against primary alveolar echinococcosis and could be used in vaccine development.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 杉本 千尋


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学