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Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis

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Title: Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis
Authors: Dang, Zhisheng Browse this author
Yagi, Kinpei Browse this author
Oku, Yuzaburo Browse this author →KAKEN DB
Kouguchi, Hirokazu Browse this author →KAKEN DB
Kajino, Kiichi Browse this author →KAKEN DB
Watanabe, Junichi Browse this author
Matsumoto, Jun Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Wakaguri, Hiroyuki Browse this author
Toyoda, Atsushi Browse this author
Sugimoto, Chihiro Browse this author →KAKEN DB
Keywords: Echinococcus multilocularis
Tetraspanin
Vaccine
Oncosphere
Primary alveolar echinococcosis
Issue Date: 9-Dec-2009
Publisher: Elsevier Ltd.
Journal Title: Vaccine
Volume: 27
Issue: 52
Start Page: 7339
End Page: 7345
Publisher DOI: 10.1016/j.vaccine.2009.09.045
PMID: 19782112
Abstract: Echinococcus multilocularis causes an important zoonotic cestode disease. The metacestocle stage proliferates in the liver of intermediate hosts including human and rodents and forms multiple cysts. Recently, members of a transmembrane protein tetraspanin (TSP) family have been used as vaccines against schistosomosis, or as diagnostic antigens for cysticercosis. In this study, seven tetraspanins of E. multilocularis, designated as TSP1 to TSP7, were evaluated for their protective potential against primary alveolar echinococcosis. The large extracellular loop (LEL) region of these tetraspanins was cloned from a full-length enriched cDNA library of E. multilocularis metacestodes and expressed in Escherichia coli as a fusion protein with thioredoxin. Recombinant TSPs were applied as vaccines against an E. multilocularis primary experimental infection in BALB/c mice. Cyst lesions in the livers of vaccinated and non-vaccinated mice were counted. The cyst lesion reduction rates induced by the seven tetraspanins in vaccinated vis-à-vis non-vaccinated mice were: 87.9%, 65.8%, 85.1%, 66.9%, 73.7%, 72.9% and 37.6%. Vaccination conferred protective rates to mice ranging from 0% (TSP5, 6, 7) to maximally 33% (TSP1, 3). The results indicated that recombinant tetraspanins have varying protective effects against primary alveolar echinococcosis and could be used in vaccine development.
Type: article (author version)
URI: http://hdl.handle.net/2115/42601
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 杉本 千尋

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