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工業用ホスホリパーゼA2による高度不飽和脂肪酸含有リン脂質の合成
Title: | 工業用ホスホリパーゼA2による高度不飽和脂肪酸含有リン脂質の合成 |
Other Titles: | Polyunsaturated Fatty Phosphatide Synthesis by Industrial Phospholipase A2 |
Authors: | 細川, 雅史1 Browse this author →KAKEN DB | 高橋, 是太郎2 Browse this author →KAKEN DB | 羽田野, 六男3 Browse this author →KAKEN DB | 江木, 衷4 Browse this author |
Authors(alt): | Hosokawa, Masashi1 | Takahashi, Koretaro2 | Hatano, Mutsuo3 | Egi, Makoto4 |
Issue Date: | 1991 |
Publisher: | 日本食品工業学会 |
Journal Title: | 日本食品工業学会誌 |
Journal Title(alt): | Journal of food science and technology |
Volume: | 38 |
Issue: | 8 |
Start Page: | 695 |
End Page: | 698 |
Abstract: | 乳化剤として市販されているリゾ型リン脂質より調製したLPC,ならびにイワシ油をケン化して調製したHUFAを基質として用い,ブタ膵臓由来ホスホリパーゼA2触媒によるHUFA含有PCの合成を行った.合成されたPCの脂肪酸の濃度は,基質として用いた脂肪酸の濃度に影響され,2位の位置に選択的に導入されることが明らかになった. | Phospholipids consist of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were synthesized by use of an industrial pancreatic phospholipase A2. Lysophosphatidylcholine (LPC) isolated from a commercial emulsifier, mixed fatty acids obtained from sardine oil, the concentrated sardine oil (EPA+DHA>60%), and 99.4% EPA were used as substrates. Optimum water content was found to 13.4%/system, and optimum ratio of LPC to mixed free fatty acids was 11:18 in weight base. When the substrate contained free EPA+DHA over 68%, the obtained phosphatidylcholine (PC) contained more than 31% of EPA+DHA. And when the substrate was 99.4% EPA, the obtained PC contained more than 45% of EPA, indicating that more than 90% of EPA was incorporated into sn-2 position of the PC molecule. |
Type: | article |
URI: | http://hdl.handle.net/2115/42697 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 是太郎
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