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Title: 工業用ホスホリパーゼA2による高度不飽和脂肪酸含有リン脂質の合成
Other Titles: Polyunsaturated Fatty Phosphatide Synthesis by Industrial Phospholipase A2
Authors: 細川, 雅史1 Browse this author →KAKEN DB
高橋, 是太郎2 Browse this author →KAKEN DB
羽田野, 六男3 Browse this author →KAKEN DB
江木, 衷4 Browse this author
Authors(alt): Hosokawa, Masashi1
Takahashi, Koretaro2
Hatano, Mutsuo3
Egi, Makoto4
Issue Date: 1991
Publisher: 日本食品工業学会
Journal Title: 日本食品工業学会誌
Journal Title(alt): Journal of food science and technology
Volume: 38
Issue: 8
Start Page: 695
End Page: 698
Abstract: 乳化剤として市販されているリゾ型リン脂質より調製したLPC,ならびにイワシ油をケン化して調製したHUFAを基質として用い,ブタ膵臓由来ホスホリパーゼA2触媒によるHUFA含有PCの合成を行った.合成されたPCの脂肪酸の濃度は,基質として用いた脂肪酸の濃度に影響され,2位の位置に選択的に導入されることが明らかになった.
Phospholipids consist of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were synthesized by use of an industrial pancreatic phospholipase A2. Lysophosphatidylcholine (LPC) isolated from a commercial emulsifier, mixed fatty acids obtained from sardine oil, the concentrated sardine oil (EPA+DHA>60%), and 99.4% EPA were used as substrates. Optimum water content was found to 13.4%/system, and optimum ratio of LPC to mixed free fatty acids was 11:18 in weight base. When the substrate contained free EPA+DHA over 68%, the obtained phosphatidylcholine (PC) contained more than 31% of EPA+DHA. And when the substrate was 99.4% EPA, the obtained PC contained more than 45% of EPA, indicating that more than 90% of EPA was incorporated into sn-2 position of the PC molecule.
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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