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Hokkaido University Collection of Scholarly and Academic Papers >
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Hydrolysis and Synthesis of Icosapentaenoic Acid-Docosahexaenoic Acid Rich Oil by Lipase TOYO (Chromobacterium viscosum)
| Title: | Hydrolysis and Synthesis of Icosapentaenoic Acid-Docosahexaenoic Acid Rich Oil by Lipase TOYO (Chromobacterium viscosum) |
| Other Titles: | リパーゼTOYO (Chromobacterium viscosum) によるイコサペンタエン酸とドコサヘキサエン酸含有油脂の加水分解と合成 |
| Authors: | Osada, Kyoichi1 Browse this author | | Takahashi, Koretaro2 Browse this author →KAKEN DB | | Hatano, Mutsuo3 Browse this author →KAKEN DB |
| Authors(alt): | 長田, 恭一1 | | 高橋, 是太郎2 | | 羽田野, 六男3 |
| Issue Date: | 20-Jan-1990 |
| Publisher: | 日本油化学協会 |
| Journal Title: | 油化学 |
| Journal Title(alt): | Journal of Japan Oil Chemist's Society |
| Volume: | 39 |
| Issue: | 1 |
| Start Page: | 50 |
| End Page: | 52 |
| Publisher DOI: | 10.5650/jos1956.39.50 |
| Abstract: | The hydrolysis and synthesis of icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) rich oil by Lipase TOYO (Chromobacterium viscosum) were conducted. EPA and DHA were found to be released from the glycerol moiety at a constant rate, and were incorporated, by the reverse reaction, into the glycerol moiety using Lipase TOYO. The optimum water content and enzyme amount for synthesis of triglyceride were 0.93% and 200 units respectively. Under these conditions, using pure EPA as well as DHA as the substrate, 91.3% of EPA and 94.9% of DHA were incorporated into the glycerol moiety. | | イコサペンタエン酸 (EPA)，ドコサヘキサエン酸 (DHA) を多く含む油脂の加水分解，合成について検討した。Lipase TOYO (Chromobacterium viscosum) により，いずれの反応も比較的容易に進行し，本酵素によりこれらの脂肪酸を含む油脂の改質が可能であることが明らかとなった。あらかじめオレイン酸とグリセリンによりトリグリセリド (TG) を合成するための至適条件を求めたところ，反応系中の水分量が0.93%，酵素量が200Unitのときが最良であると判断され，また，同条件下でのEPA，DHAとグリセリンからのTGの合成率は，EPAで91.3%，DHAでは94.9%に達した。 |
| Description: | LETTER |
| Type: | article |
| URI: | http://hdl.handle.net/2115/42708 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 是太郎
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