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微生物由来固定化リパーゼによるいわし油の改質

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Title: 微生物由来固定化リパーゼによるいわし油の改質
Other Titles: Modification of Sardine Oil by Immobilized Bacterial Lipase
Authors: 長田, 恭一1 Browse this author
高橋, 是太郎2 Browse this author →KAKEN DB
羽田野, 六男3 Browse this author →KAKEN DB
Authors(alt): Osada, Kyoichi1
Takahashi, Koretaro2
Hatano, Mutsuo3
Issue Date: 20-Jul-1990
Publisher: 日本油化学協会
Journal Title: 油化学
Journal Title(alt): Journal of Japan Oil Chemist's Society
Volume: 39
Issue: 7
Start Page: 467
End Page: 471
Abstract: Determination was made of the rates of icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) incorporation into triolein and sardine oil triglyceride (TG). Three kinds of lipases (Lipase OF from Candida cyrindracea, Lipase A from Aspergillus sp. and Lipase TOYO from Chromobacterium viscosum) were first immobilized onto Celite 545. A preliminary study was then performed to determine optimum conditions for acidolysis by conducting this process between triolein and palmitic acid, using these immobilized lipases including LipozymeTH (Mucor miehei lipase was immobilized on a macroporous anion exchange resin). Optimum water contents for Lipase OF, Lipase A, Lipozyme, and Lipase TOYO reaction systems were 20.38%, 10.24%, 6.38~10.38%, and 5.18%, respectively, and the optimum immobilized lipase amount was about 250 mg for any of the four lipases. Under the above conditions, the acidolysis of triolein between pure EPA and then DHA was carried out. EPA incorporation rate was found highest in the system using Lipase OF (25.09%), whereas in the case of DHA, it was highest in the system using Lipase TOYO (23.02%). Finally, acidolysis of sardine oil TG with an EPA, DHA concentrated free fatty acid mixture (EPA+DHA : 68.83%) was carried out and the results examined. As a result of the low incorporation rates of EPA and DHA into the original sardine oil TG, the percentage of DHA in the reactant TG doubled in the reaction systems using Lipase TOYO and Lipozyme.
Description: 報文
Type: article
URI: http://hdl.handle.net/2115/42709
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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