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Effect of Phospholipids Containing Docosahexaenoic Acid on Differentiation and Growth of HL-60 Human Promyelocytic Leukemia Cells

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Title: Effect of Phospholipids Containing Docosahexaenoic Acid on Differentiation and Growth of HL-60 Human Promyelocytic Leukemia Cells
Other Titles: HL-60ヒト前骨髄性白血病細胞の分化と増殖に及ぼすドコサヘキサエン酸結合型リン脂質の影響
Authors: Tochisawa, Kaoru1 Browse this author
Hosokawa, Masashi2 Browse this author →KAKEN DB
Kurihara, Hideyuki3 Browse this author →KAKEN DB
Kohno, Hiroyuki4 Browse this author
Odashima, Shizuo5 Browse this author
Takahashi, Koretaro6 Browse this author →KAKEN DB
Authors(alt): 栃澤, 馨1
細川, 雅史2
栗原, 秀幸3
甲野, 裕之4
小田島, 粛夫5
高橋, 是太郎6
Keywords: docosahexaenoic acid-containing phospholipid
differentiation
cell growth
HL-60
Issue Date: 20-Apr-1997
Publisher: 日本油化学会
Journal Title: 日本油化学会誌
Journal Title(alt): Journal of Japan Oil Chemists' Society
Volume: 46
Issue: 4
Start Page: 383
End Page: 390
Abstract: Effects of docosahexaenoic acid (DHA)-containing phospholipids (DHA-PLs) on retionic acid (RA)-induced cell differentiation and growth inhibition of the human promyelocytic leukemia cell line, HL-60 were investigated. Cell differentiation judged from NBT reduction assay reached to 50~60% by incubation with 12.5μM DHA-containing phosphatidylcholines (DHA-PCs) prior to the addition of RA. Differentiation without DHA-PCs remained at 39.2%. By prior incubation with DHA-containing phosphatidylethanolamines (DHA-PEs), differentiation was 70% at 12.5μM. DHA-PEs induced NBT reducing activity to as much as 20~30%, and inhibited cell growth in the absence of RA. Activity for promoting cell differentiation differed according to DHA-PL molecular species. 1-Palmitoyl (or oleoyl)-2-docosahexaenoyl-sn-glycero-3-phosphocholine (or phosphoethanolamine) was found most effective molecular species for enhancing RA-induced cell differentiation.
ヒト前骨髄性白血病細胞(HL-60細胞)の分化と増殖に及ぼすドコサヘキサエン酸 (DHA) 結合型リン脂質の影響について検討した。分化誘導剤であるレチノイン酸 (RA) の単独添加に比べ,DHA結合型ホスファチジルコリンでインキュベーションした後にRAを添加することで分化率が有意に上昇した。このようなRAの分化誘導能に対する促進効果は,sn-1位がパルミチン酸又はオレイン酸,sn-2位がDHAである分子種において顕著であった。同一分子種のホスファチジルエタノールアミン (PE) の添加では,ホスファチジルコリンに比べより大きな分化誘導促進効果がみられ,DHA結合型PEのみを単独添加した場合においても,強い細胞増殖抑制作用とNBT還元能の上昇がみられた。以上の結果より,HL-60細胞の分化誘導および増殖抑制に対して,1-palmitoyl又は1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamineが最も効果的であった。
Type: article
URI: http://hdl.handle.net/2115/42714
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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