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Butyrate-Induced Growth Inhibition and Differentiation in Caco-2 Cells with Hybrid Liposomes
Title: | Butyrate-Induced Growth Inhibition and Differentiation in Caco-2 Cells with Hybrid Liposomes |
Authors: | Hossain, Zakir Browse this author | Kurihara, Hideyuki Browse this author →KAKEN DB | Hosokawa, Masashi Browse this author →KAKEN DB | Takahashi, Koretaro Browse this author →KAKEN DB |
Keywords: | hybrid liposome | growth inhibition | differentiation | Caco-2 cells |
Issue Date: | 2005 |
Publisher: | Japan oil chemists' society |
Journal Title: | Journal of Oleo Science |
Volume: | 54 |
Issue: | 5 |
Start Page: | 281 |
End Page: | 288 |
Abstract: | Hybrid liposomes were prepared by mixing glycerophospholipids with glyceroglycolipids. The effects of hybrid liposomes on Caco-2 cells were investigated. Growth inhibition of cells was determined with sodium 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium dye reduction assay. Alkaline phosphatase activity was measured for assessing the cell differentiation induced by sodium butyrate (NaBT). Growth of Caco-2 cells was inhibited at 48 h after addition of all four types hybrid liposomes (PC/DGDG, PC/SQDG, PS/DGDG and PS/SQDG) while at 72 h growth inhibition was significant for all treatments except the hybrid liposome of phosphatidylcholine and digalactosyldiacylglycerol under concentrations of 50 μM glycerophospholipids with 50 μM glyceroglycolipids. Alkaline phosphatase activity of Caco-2 cells increased significantly when cells were treated in combination of hybrid liposomes and NaBT at 72 h, indicating that hybrid liposomes enhanced cell differentiation induced with NaBT. |
Type: | article |
URI: | http://hdl.handle.net/2115/42751 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 是太郎
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