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北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第60巻 第1号 >

Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood

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Title: Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood
Authors: Kobayashi, Tetsuya Browse this author
Yamazaki, Koji Browse this author →KAKEN DB
Bagenda, Dominic K. Browse this author
Kawai, Yuji Browse this author →KAKEN DB
Keywords: Bacteriocin
Pediocin
Pediococcus
Medium components
Culture condition
Optimization
Issue Date: 9-Mar-2010
Publisher: 北海道大学大学院水産科学研究院
Journal Title: 北海道大学水産科学研究彙報
Journal Title(alt): Bulletin of Fisheries Sciences, Hokkaido University
Volume: 60
Issue: 1
Start Page: 5
End Page: 12
Abstract: Growth requirements for maximum production of pediocin Iz3.13 by Pediococcus pentosaceus Iz3.13 were studied. Physical and chemical parameters that enhanced pediocin production included incubation in static broth at 30℃, with an initial pH of 4.5. Optimum nutritional conditions that enhanced pediocin Iz3.13 production were 3.5% Trypticase peptone, 3.5% Phytone peptone or 5.0% Proteose peptone No.3 as nitrogen sources, and glucose or maltose as carbon sources. Supplementing growth media with beef extract and MgCl2 also enhanced production. This study proposes an optimized growth medium containing 5.0% Proteose peptone No.3, 3.5% Phytone peptone, 2.5% glucose, 1.5% MgCl2, 3.0% beef extract and 0.25% K2HPO4. This medium significantly enhanced pediocin Iz3.13 production (5.1 fold increase) as compared to a basal medium.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/42764
Appears in Collections:北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第60巻 第1号

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