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A simple turbidimetric method for monitoring the inhibition of tRNA-dependent amidotransferase GatCAB

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タイトル: A simple turbidimetric method for monitoring the inhibition of tRNA-dependent amidotransferase GatCAB
著者: Chatani, Miho 著作を一覧する
Tanaka, Michiko 著作を一覧する
Nakamura, Akiyoshi 著作を一覧する
Takesue, Nobuchika 著作を一覧する
Tanaka, Isao 著作を一覧する
Asano, Kozo 著作を一覧する
キーワード: Escherichia coli
Inhibitor
Non-discriminating glutamyl-tRNA
tRNA-dependent amidotransferase GatCAB
Turbidimetric assay
発行日: 2010年 2月
出版者: Elsevier
誌名: Journal of Microbiological Methods
巻: 80
号: 2
開始ページ: 117
終了ページ: 122
出版社 DOI: 10.1016/j.mimet.2009.10.014
抄録: In eubacteria that lack glutaminyl-tRNA synthetase (GlnRS), a tRNA-dependent amidotransferase (GatCAB) recognizes mischarged Glu-tRNA^[Gln] and converts it into Gln-tRNA^[Gln]. An inhibitor specific for GatCAB could therefore act as an antibiotic with a novel mode of action against multidrug-resistant bacteria such as Staphylococcus strains. However, there is no rapid, simple and efficient screening method for specifically monitoring the inhibition of GatCAB activity. We have focused on developing a simple system for monitoring the inhibition of GatCAB activity using Escherichia coli Top10 co-expressing the ndGluRS and GatCAB genes from Staphylococcus aureus Mu50. First, growth repression was confirmed by introducing ndgluRS from S. aureus Mu50 into E. coli. Then, we verified that co-expression of the gatCAB operon alleviated growth repression in the host E. coli. The screening system consisted of these two transformants and non-expressing E. coli Top10. The transformant harbors both ndGluRS gene and GatCAB operon could be co-expressed in the presence and in the absence of chemical compound of interest. Since there is no inhibitor that inactivates GatCAB activity, we expressed two inactive GatCAB deletion variants, GatCAB^[Δ10] and GatCAB^[ΔCHD] together with ndGluRS in E. coli Top10. The expressed E. coli showed repressed growth as well as ndGluRS expressed. These results indicate that if GatCAB activity is inhibited in this co-expressed E. coli, the inhibition can be monitored by the decrease in O.D. of the co-expressed E. coli.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/42768
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 田中 みち子

 

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