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A simple turbidimetric method for monitoring the inhibition of tRNA-dependent amidotransferase GatCAB

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Title: A simple turbidimetric method for monitoring the inhibition of tRNA-dependent amidotransferase GatCAB
Authors: Chatani, Miho Browse this author
Tanaka, Michiko Browse this author
Nakamura, Akiyoshi Browse this author
Takesue, Nobuchika Browse this author
Tanaka, Isao Browse this author
Asano, Kozo Browse this author →KAKEN DB
Keywords: Escherichia coli
Non-discriminating glutamyl-tRNA
tRNA-dependent amidotransferase GatCAB
Turbidimetric assay
Issue Date: Feb-2010
Publisher: Elsevier
Journal Title: Journal of Microbiological Methods
Volume: 80
Issue: 2
Start Page: 117
End Page: 122
Publisher DOI: 10.1016/j.mimet.2009.10.014
PMID: 19879905
Abstract: In eubacteria that lack glutaminyl-tRNA synthetase (GlnRS), a tRNA-dependent amidotransferase (GatCAB) recognizes mischarged Glu-tRNA^[Gln] and converts it into Gln-tRNA^[Gln]. An inhibitor specific for GatCAB could therefore act as an antibiotic with a novel mode of action against multidrug-resistant bacteria such as Staphylococcus strains. However, there is no rapid, simple and efficient screening method for specifically monitoring the inhibition of GatCAB activity. We have focused on developing a simple system for monitoring the inhibition of GatCAB activity using Escherichia coli Top10 co-expressing the ndGluRS and GatCAB genes from Staphylococcus aureus Mu50. First, growth repression was confirmed by introducing ndgluRS from S. aureus Mu50 into E. coli. Then, we verified that co-expression of the gatCAB operon alleviated growth repression in the host E. coli. The screening system consisted of these two transformants and non-expressing E. coli Top10. The transformant harbors both ndGluRS gene and GatCAB operon could be co-expressed in the presence and in the absence of chemical compound of interest. Since there is no inhibitor that inactivates GatCAB activity, we expressed two inactive GatCAB deletion variants, GatCAB^[Δ10] and GatCAB^[ΔCHD] together with ndGluRS in E. coli Top10. The expressed E. coli showed repressed growth as well as ndGluRS expressed. These results indicate that if GatCAB activity is inhibited in this co-expressed E. coli, the inhibition can be monitored by the decrease in O.D. of the co-expressed E. coli.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 みち子

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