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Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes

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タイトル: Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes
著者: Yamamoto, Takashi 著作を一覧する
Sugawara, Mitsuru 著作を一覧する
Kikukawa, Takashi 著作を一覧する
Miyauchi, Seiji 著作を一覧する
Yamaguchi, Masahiro 著作を一覧する
Tero, Atsushi 著作を一覧する
Takagi, Seiji 著作を一覧する
Nakagaki, Toshiyuki 著作を一覧する
キーワード: Diffusion
Mathematical model
Nucleoside transporter
発行日: 2010年 3月
出版者: Elsevier
誌名: Biophysical Chemistry
巻: 147
号: 1-2
開始ページ: 59
終了ページ: 65
出版社 DOI: 10.1016/j.bpc.2009.12.012
抄録: Transport across the cell membrane is crucial in drug delivery. However, the process is complicated because nucleoside derivatives that are commonly used as anti-viral drugs are transported through two different types of specific transporters: concentrative transporters and equilibrative transporters. Cross-disciplinary approaches involving both biological experiments and theoretical considerations are therefore necessary to study the transport of nucleoside analogues such as ribavirin. Here we constructed an experimental model system using the Xenopus laevis oocyte that expressed examples of both types of transporters: human concentrative nucleoside transporter 3 and human equilibrative transporter 1. We also performed a kinetic study. Experimental results showed that the transport of ribavirin could be reduced by inhibiting one of the two types of transporters, which seems to be counterintuitive. We therefore designed a simple mathematical model of the dynamics of ribavirin uptake and analyzed the model behaviors using a numerical simulation. The theoretical results reproduced the experimentally observed phenomena and suggested a possible mechanism for the process. Based on this mechanism, we predicted some potential methods for the effective uptake of ribavirin from a dynamics point of view.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 中垣 俊之


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