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PAX4 has the potential to function as a tumor suppressor in human melanoma

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タイトル: PAX4 has the potential to function as a tumor suppressor in human melanoma
著者: Hata, Shinya 著作を一覧する
Hamada, Jun-Ichi 著作を一覧する
Maeda, Kazuhiko 著作を一覧する
Murai, Taichi 著作を一覧する
Tada, Mitsuhiro 著作を一覧する
Furukawa, Hiroshi 著作を一覧する
Tsutsumida, Arata 著作を一覧する
Saito, Akira 著作を一覧する
Yamamoto, Yuhei 著作を一覧する
Moriuchi, Tetsuya 著作を一覧する
発行日: 2008年11月
出版者: Spandidos Publications
誌名: International Journal of Oncology
巻: 33
号: 5
開始ページ: 1065
終了ページ: 1071
抄録: We hypothesize that dysregulated expression levels of the developmental regulatory genes in the adult body result in tumor development and malignant progression. PAX genes discovered as human orthologous genes of Drosophila 'paired' encode transcription factors, which control the expression of target genes to go on along the program of development. In this study, we first quantified expression of 9 PAX genes in human nevus pigmentosus tissues, melanoma tissues and melanoma cell lines by the real-time reverse transcription-PCR method. As a result, we found that the expression levels of PAX4 and PAX9 were extremely low in melanoma tissues and cell lines compared to nevus pigmentosus tissues. We then established melanoma cells overexpressing PAX4 and examined roles of PAX4 in cell growth. PAX4-overexpression reduced in vitro cell growth of human melanoma C8161 and MeWo cells. BrdU-uptake assay and cell cycle analysis by flow cytometry indicated that the retardation of cell proliferation by PAX4-overexpression was due to decreased DNA synthesis and cell cycle arrest at the G0/G1 phase. Furthermore, treatment of C8161 and MeWo cells with 5-azacytidine, a DNA demethylating agent, induced the expression of PAX4, suggesting that DNA methylation repressed the PAX4 gene expression in human melanoma. These results suggest that PAX4 functions as a potent tumor suppressor.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 浜田 淳一


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