|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Visualization of Nuclear Localization of Transcription Factors with Cyan and Green Fluorescent Proteins in the Red Alga Porphyra yezoensis
| Title: | Visualization of Nuclear Localization of Transcription Factors with Cyan and Green Fluorescent Proteins in the Red Alga Porphyra yezoensis |
| Authors: | Uji, Toshiki Browse this author →KAKEN DB | | Takahashi, Megumu Browse this author | | Saga, Naotsune Browse this author →KAKEN DB | | Mikami, Koji Browse this author →KAKEN DB |
| Keywords: | Transcription factor | | nuclear localization | | fluorescent protein | | transient gene expression | | Porphyra yezoensis |
| Issue Date: | Apr-2010 |
| Publisher: | Springer |
| Journal Title: | Marine Biotechnology |
| Volume: | 12 |
| Issue: | 2 |
| Start Page: | 150 |
| End Page: | 159 |
| Publisher DOI: | 10.1007/s10126-009-9210-5 |
| PMID: | 19593603 |
| Abstract: | Transcription factors play a central role in expression of genomic information in all organisms. The objective of our study is to analyze the function of transcription factors in red algae. One way to analyze transcription factors in eukaryotic cells is to study their nuclear localization, as reported for land plants and green algae using fluorescent proteins. There is, however, no report documenting subcellular localization of transcription factors from red algae. In the present study, using the marine red alga Porphyra yezoensis, we confirmed for the first time successful expression of humanized fluorescent proteins (ZsGFP and ZsYFP) from a reef coral Zoanthus sp. and land plant-adapted sGFP(S65T) in gametophytic cells comparable to expression of AmCFP. Following molecular cloning and characterization of transcription factors DP-E2F-like 1 (PyDEL1), transcription elongation factor 1 (PyElf1) and multiprotein bridging factor 1 (PyMBF1), we then demonstrated that ZsGFP and AmCFP can be used to visualize nuclear localization of PyElf1 and PyMBF1. This is the first report to perform visualization of subcellular localization of transcription factors as genome-encoded proteins in red algae. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/42987 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 三上 浩司
|
