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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Semi-automated protein crystal mounting device for the sulfur single-wavelength anomalous diffraction method
| Title: | Semi-automated protein crystal mounting device for the sulfur single-wavelength anomalous diffraction method |
| Authors: | Kitago, Yu Browse this author | | Watanabe, Nobuhisa Browse this author | | Tanaka, Isao Browse this author |
| Issue Date: | Apr-2010 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Journal of Applied Crystallography |
| Volume: | 43 |
| Issue: | 2 |
| Start Page: | 341 |
| End Page: | 346 |
| Publisher DOI: | 10.1107/S0021889809054272 |
| Abstract: | Use of longer-wavelength X-rays has advantages for the detection of small anomalous signals from light atoms, such as sulfur, in protein molecules. However, the accuracy of the measured diffraction data decreases at longer wavelengths because of the greater X-ray absorption. The capillary-top mounting method (formerly the loopless mounting method) makes it possible to eliminate frozen solution around the protein crystal and reduces systematic errors in the evaluation of small anomalous differences. However, use of this method requires custom-made tools and a large amount of skill. Here, the development of a device that can freeze the protein crystal semi-automatically using the capillary-top mounting method is described. This device can pick up the protein crystal from the crystallization drop using a micro-manipulator, and further procedures, such as withdrawal of the solution around the crystal by suction and subsequent flash freezing of the protein crystal, are carried out automatically. This device makes it easy for structural biologists to use the capillary-top mounting method for sulfur single-wavelength anomalous diffraction phasing using longer-wavelength X-rays. |
| Description: | cryocrystallography papers |
| Type: | article |
| URI: | http://hdl.handle.net/2115/43006 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡邉 信久
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