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Difructose anhydride III promotes iron absorption in the rat large intestine

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Title: Difructose anhydride III promotes iron absorption in the rat large intestine
Authors: Hara, Hiroshi Browse this author →KAKEN DB
Onoshima, Satoshi Browse this author
Nakagawa, Chie Browse this author
Keywords: Iron absorption
Difructose anhydride III
Small intestine
Divalent cation transporter-1
Issue Date: Jan-2010
Publisher: Elsevier
Journal Title: Nutrition
Volume: 26
Issue: 1
Start Page: 120
End Page: 127
Publisher DOI: 10.1016/j.nut.2009.05.024
PMID: 20005467
Abstract: Objectives: We examined the intestinal segment responsible for and the mechanisms involved in the nondigestible disaccharide difructose anhydride III (DFA) III-mediated enhancement of iron absorption. Methods: Net iron and calcium absorption (%) were observed in male SD rats fed an AIN93G-based diet with or without DFAIII (30 g/kg diet) for 2 weeks after cecectomy or laparotomy (Exp. 1). The absorption rates of calcium and iron from ligated jejunal loops (Exp. 2) or ligated cecal sacs (Exp. 3) in anesthetized rats fed a control or DFA diet were observed with or without the addition of DFAIII to the instilled mucosal fluid. Levels of the major iron transporter DMT-1 mRNA per 28S rRNA were evaluated by real-time PCR in the intestinal mucosa. Results: Net absorption (%) of iron and calcium was higher in the DFA group than in the DFA-free group in laparotomized rats. The enhancement of iron absorption was almost and calcium absorption was partly abolished by cecectomy. The absorption rate of calcium, but not iron, from the jejunal loops was increased by the addition of DFAIII (100 mmol/L). Iron absorption was clearly higher in the ligated cecal sacs of the DFAIII-fed group than in the DFA-free group with or without the mucosal addition of DFAIII. DMT-1 mRNA level per 28S rRNA was maintained in the cecal mucosa through large increases in cecal size due to DFAIII feeding. Conclusion: DFAIII-induced increases in iron absorption are the result of increased cecal iron absorptive capacity through expansion of the cecal mucosa maintaining DMT-1 mRNA expression.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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