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Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable-dependent Vesicle Transport in Yeast

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Title: Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable-dependent Vesicle Transport in Yeast
Authors: Yamamoto, Takaharu Browse this author →KAKEN DB
Mochida, Junko Browse this author
Kadota, Jun Browse this author
Takeda, Miyoko Browse this author
Bi, Erfei Browse this author
Tanaka, Kazuma Browse this author
Issue Date: 1-Apr-2010
Publisher: American Society for Cell Biology
Journal Title: Molecular Biology of the Cell
Volume: 21
Issue: 7
Start Page: 1237
End Page: 1252
Publisher DOI: 10.1091/mbc.E09-05-0412
Abstract: The assembly of filamentous actin is essential for polarized bud growth in budding yeast. Actin cables, which are assembled by the formins Bni1p and Bnr1p, are thought to be the only actin structures that are essential for budding. However, we found that formin or tropomyosin mutants, which lack actin cables, are still able to form a small bud. Additional mutations in components for cortical actin patches, which are assembled by the Arp2/3 complex to play a pivotal role in endocytic vesicle formation, inhibited this budding. Genes involved in endocytic recycling were also required for small-bud formation in actin cable-less mutants. These results suggest that budding yeast possesses a mechanism that promotes polarized growth by local recycling of endocytic vesicles. Interestingly, the type V myosin Myo2p, which was thought to use only actin cables to track, also contributed to budding in the absence of actin cables. These results suggest that some actin network may serve as the track for Myo2p-driven vesicle transport in the absence of actin cables or that Myo2p can function independent of actin filaments. Our results also show that polarity regulators including Cdc42p were still polarized in mutants defective in both actin cables and cortical actin patches, suggesting that the actin cytoskeleton does not play a major role in cortical assembly of polarity regulators in budding yeast.
Rights: © 2010 by The American Society for Cell Biology
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 一馬

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