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Extrusion of Na,K-ATPase and transferrin receptor with lipid raft-associated proteins in different populations of exosomes during reticulocyte maturation in dogs

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/jjvr.58.1.17

Title: Extrusion of Na,K-ATPase and transferrin receptor with lipid raft-associated proteins in different populations of exosomes during reticulocyte maturation in dogs
Authors: Komatsu, Tomohiko Browse this author
Arashiki, Nobuto Browse this author
Otsuka, Yayoi Browse this author
Sato, Kota Browse this author
Inaba, Mutsumi Browse this author
Keywords: dog
exosome
Na,K-ATPase
reticulocyte
stomatin
Issue Date: May-2010
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 58
Issue: 1
Start Page: 17
End Page: 27
Abstract: The present study characterizes canine reticulocyte exosomes. Exosomes are small membrane vesicles involved in membrane remodeling that are released from reticulocytes during the final maturation step of red blood cells. The vesicles collected from reticulocyte culture supernatants by differential centrifugation contained major exosomal proteins including heat shock protein cognate 70 (Hsc70) and transferrin receptors (TfR), consistent with the definition of the exosome. In addition, the Na,K-ATPase α-subunit and stomatin, a lipid raft-associated protein, were extruded by the exosome pathway, possibly leading to the absence of these proteins in erythrocytes, while the major protein constituents of erythrocyte membranes, spectrin and band 3 were retained in reticulocytes and not expelled into exosomes. The Na,K-ATPase α-subunit, as well as TfR and about half of the stomatin contained in exosomes, was obtained in a detergent-soluble fraction that was distinct from the lipid raft microdomain. Moreover, Na,K-ATPase and a portion of stomatin were distributed differently to Hsc70, TfR, stomatin, and ganglioside Gm1 in vesicles separated with sucrose density gradient centrifugation. These results demonstrate that a heterogeneous group of exosomes participates in the loss of Na,K-ATPase and membrane remodeling during reticulocyte maturation in dogs.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/43054
Appears in Collections:Japanese Journal of Veterinary Research > Volume 58, Number 1

Submitter: 獣医学部図書室

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