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Three types of proteinases in Japanese common squid Todarodes pacificus hepatopancreas as studied by using carp myofibrils as substrate
Title: | Three types of proteinases in Japanese common squid Todarodes pacificus hepatopancreas as studied by using carp myofibrils as substrate |
Authors: | Tian, Yuanyong Browse this author | Umezawa, Emi Browse this author | Duan, Rui Browse this author | Konno, Kunihiko Browse this author →KAKEN DB |
Keywords: | Cysteine-proteinase | Denaturation | Hepatopancreas | Japanese common squid | Myofibrils | Proteinase |
Issue Date: | Mar-2010 |
Publisher: | Springer Japan |
Journal Title: | Fisheries Science |
Volume: | 76 |
Issue: | 2 |
Start Page: | 365 |
End Page: | 373 |
Publisher DOI: | 10.1007/s12562-009-0206-x |
Abstract: | Three types of proteinases, namely cysteine-, metallo-, and serine-proteinases, were found in squid hepatopancreas by studying the inhibition spectra using carp myofibril as substrate. The cysteine-, metallo-, and serine-types showed the highest activities at 50℃, 35℃, and 40℃, respectively. The optimal pHs were pH 5, pH 7, and pH 9 for the cysteine-, metallo-, and serine-types, respectively. When assayed at 20℃ and pH 7.5, the metallo-type showed the highest activity. The metallo-type was characterized by a high selectivity in the digestion of myosin. Among the three enzymes, the cysteine-type was found to be the most stable against thermal and acid treatments. Heat treated myofibrils were more susceptible to cysteine- and serine-types, but less susceptible to the metallo-type. Acid treatment of myofibrils also enhanced the digestibility by cysteine type. The results indicated that the cysteine-type seemed to be the most suitable enzyme to produce peptides from denatured myofibrils by their random digestion. |
Rights: | The original publication is available at www.springerlink.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/43064 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 今野 久仁彦
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