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Three types of proteinases in Japanese common squid Todarodes pacificus hepatopancreas as studied by using carp myofibrils as substrate

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Title: Three types of proteinases in Japanese common squid Todarodes pacificus hepatopancreas as studied by using carp myofibrils as substrate
Authors: Tian, Yuanyong Browse this author
Umezawa, Emi Browse this author
Duan, Rui Browse this author
Konno, Kunihiko Browse this author →KAKEN DB
Keywords: Cysteine-proteinase
Denaturation
Hepatopancreas
Japanese common squid
Myofibrils
Proteinase
Issue Date: Mar-2010
Publisher: Springer Japan
Journal Title: Fisheries Science
Volume: 76
Issue: 2
Start Page: 365
End Page: 373
Publisher DOI: 10.1007/s12562-009-0206-x
Abstract: Three types of proteinases, namely cysteine-, metallo-, and serine-proteinases, were found in squid hepatopancreas by studying the inhibition spectra using carp myofibril as substrate. The cysteine-, metallo-, and serine-types showed the highest activities at 50℃, 35℃, and 40℃, respectively. The optimal pHs were pH 5, pH 7, and pH 9 for the cysteine-, metallo-, and serine-types, respectively. When assayed at 20℃ and pH 7.5, the metallo-type showed the highest activity. The metallo-type was characterized by a high selectivity in the digestion of myosin. Among the three enzymes, the cysteine-type was found to be the most stable against thermal and acid treatments. Heat treated myofibrils were more susceptible to cysteine- and serine-types, but less susceptible to the metallo-type. Acid treatment of myofibrils also enhanced the digestibility by cysteine type. The results indicated that the cysteine-type seemed to be the most suitable enzyme to produce peptides from denatured myofibrils by their random digestion.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/43064
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 今野 久仁彦

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