HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >

pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis

Files in This Item:
295-2_p170-176.pdf263.15 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/43086

Title: pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis
Authors: Orikasa, Yoshitake Browse this author
Tanaka, Mika Browse this author
Sugihara, Shinji Browse this author
Hori, Ryuji Browse this author
Nishida, Takanori Browse this author
Ueno, Akio Browse this author
Morita, Naoki Browse this author
Yano, Yutaka Browse this author
Yamamoto, Kouhei Browse this author
Shibahara, Akira Browse this author
Hayashi, Hidenori Browse this author
Yamada, Yohko Browse this author
Yamada, Akiko Browse this author
Yu, Reiko Browse this author
Watanabe, Kazuo Browse this author
Okuyama, Hidetoshi Browse this author →KAKEN DB
Keywords: docosahexaenoic acid
eicosapentaenoic acid
Moritella marina strain MP-1
polyunsaturated fatty acid
pfaB gene
Issue Date: Jun-2009
Publisher: Blackwell Publishing
Journal Title: FEMS Microbiology Letters
Volume: 295
Issue: 2
Start Page: 170
End Page: 176
Publisher DOI: 10.1111/j.1574-6968.2009.01582.x
PMID: 19453514
Abstract: When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.
Rights: The definitive version is available at www.blackwell-synergy.com
Type: article (author version)
URI: http://hdl.handle.net/2115/43086
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University