Hokkaido University Collection of Scholarly and Academic Papers >
Research Institute for Electronic Science >
Peer-reviewed Journal Articles, etc >
Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis
Title: | Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis |
Authors: | Ito, Toshiyuki Browse this author | Oshita, Shugo Browse this author | Nakabayashi, Takakazu Browse this author | Sun, Fan Browse this author | Kinjo, Masataka Browse this author →KAKEN DB | Ohta, Nobuhiro Browse this author |
Keywords: | Fluorescence lifetime imaging (FLIM) | Apoptosis | HeLa cell | Enhanced green fluorescent protein (EGFP) | Tumor necrosis factor-α (TNF-α) | Cycloheximide |
Issue Date: | Jun-2009 |
Publisher: | Royal Society of Chemistry |
Journal Title: | Photochemical & Photobiological Sciences |
Volume: | 8 |
Issue: | 6 |
Start Page: | 763 |
End Page: | 767 |
Publisher DOI: | 10.1039/b902341k |
PMID: | 19492103 |
Abstract: | Fluorescence lifetime images of HeLa cells expressing enhanced green fluorescent protein (EGFP) have been measured, as apoptosis is induced by tumor necrosis factor-α (TNF-α) in combination with cycloheximide. The fluorescence lifetime of EGFP is found to decrease after the induction of apoptosis, indicating that the change in environment occurs around the chromophore of EGFP with the apoptosis process. The fluorescence lifetime imaging technique can be used to perform in vivo observation of cell death processes. Fluorescence lifetime measurements are useful to examine the induction of the apoptosis process, even when a morphological change of each cell cannot be observed because of a low spatial resolution. |
Rights: | Photochem. Photobiol. Sci., 2009, 8, 763-767 - Reproduced by permission of The Royal Society of Chemistry (RSC) |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/43088 |
Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 太田 信廣
|