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Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

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Title: Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis
Authors: Ito, Toshiyuki Browse this author
Oshita, Shugo Browse this author
Nakabayashi, Takakazu Browse this author
Sun, Fan Browse this author
Kinjo, Masataka Browse this author →KAKEN DB
Ohta, Nobuhiro Browse this author
Keywords: Fluorescence lifetime imaging (FLIM)
HeLa cell
Enhanced green fluorescent protein (EGFP)
Tumor necrosis factor-α (TNF-α)
Issue Date: Jun-2009
Publisher: Royal Society of Chemistry
Journal Title: Photochemical & Photobiological Sciences
Volume: 8
Issue: 6
Start Page: 763
End Page: 767
Publisher DOI: 10.1039/b902341k
PMID: 19492103
Abstract: Fluorescence lifetime images of HeLa cells expressing enhanced green fluorescent protein (EGFP) have been measured, as apoptosis is induced by tumor necrosis factor-α (TNF-α) in combination with cycloheximide. The fluorescence lifetime of EGFP is found to decrease after the induction of apoptosis, indicating that the change in environment occurs around the chromophore of EGFP with the apoptosis process. The fluorescence lifetime imaging technique can be used to perform in vivo observation of cell death processes. Fluorescence lifetime measurements are useful to examine the induction of the apoptosis process, even when a morphological change of each cell cannot be observed because of a low spatial resolution.
Rights: Photochem. Photobiol. Sci., 2009, 8, 763-767 - Reproduced by permission of The Royal Society of Chemistry (RSC)
Type: article (author version)
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 太田 信廣

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