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A novel copper(II) coordination at His186 in full-length murine prion protein

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タイトル: A novel copper(II) coordination at His186 in full-length murine prion protein
著者: Watanabe, Yasuko 著作を一覧する
Hiraoka, Wakako 著作を一覧する
Igarashi, Manabu 著作を一覧する
Ito, Kimihito 著作を一覧する
Shimoyama, Yuhei 著作を一覧する
Horiuchi, Motohiro 著作を一覧する
Yamamori, Tohru 著作を一覧する
Yasui, Hironobu 著作を一覧する
Kuwabara, Mikinori 著作を一覧する
Inagaki, Fuyuhiko 著作を一覧する
Inanami, Osamu 著作を一覧する
キーワード: Prion protein
C-terminal domain
Interspin distance
Dipolar interaction
Histidine residue
発行日: 2010年 4月 9日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 394
号: 3
開始ページ: 522
終了ページ: 528
出版社 DOI: 10.1016/j.bbrc.2010.03.003
抄録: To explore Cu(II) ion coordination by His186 in the C-terminal domain of full-length prion protein (moPrP), we utilized the magnetic dipolar interaction between a paramagnetic metal, Cu(II) ion, and a spin probe introduced in the neighborhood of the postulated binding site by the spin labeling technique (SDSL technique). Six moPrP mutants, moPrP(D143C), moPrP(Y148C), moPrP(E151C), moPrP(Y156C), moPrP(T189C), and moPrP(Y156C,H186A), were reacted with a methane thiosulfonate spin probe and a nitroxide residue (R1) was created in the binding site of each one. Line broadening of the ESR spectra was induced in the presence of Cu(II) ions in moPrP(Y148R1), moPrP(Y151R1), moPrP(Y156R1), and moPrP( T189R1) but not moPrP(D143R1). This line broadening indicated the presence of electron–electron dipolar interaction between Cu(II) and the nitroxide spin probe, suggesting that each interspin distance was within 20 Å. The interspin distance ranges between Cu(II) and the spin probes of moPrP(Y148R1), moPrP(Y151R1), moPrP(Y156R1), and moPrP(T189R1) were estimated to be 12.1 Å, 18.1 Å, 10.7 Å, and 8.4 Å, respectively. In moPrP(Y156R1,H186A), line broadening between Cu(II) and the spin probe was not observed. These results suggest that a novel Cu(II) binding site is involved in His186 in the Helix2 region of the C-terminal domain of moPrPC.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 稲波 修


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