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DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

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Title: DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma
Authors: Wang, Lei Browse this author
Nishihara, Hiroshi Browse this author →KAKEN DB
Kimura, Taichi Browse this author
Kato, Yasutaka Browse this author
Tanino, Mishie Browse this author
Nishio, Mitsufumi Browse this author
Obara, Masato Browse this author
Endo, Tomoyuki Browse this author
Koike, Takao Browse this author
Tanaka, Shinya Browse this author
Keywords: DOCK2
B cell lymphoma
Rac
ERK
Hematopoietic matignancy
Issue Date: 23-Apr-2010
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 395
Issue: 1
Start Page: 111
End Page: 115
Publisher DOI: 10.1016/j.bbrc.2010.03.148
PMID: 20350533
Abstract: DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.
Type: article (author version)
URI: http://hdl.handle.net/2115/43094
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 西原 広史

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