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DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

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タイトル: DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma
著者: Wang, Lei 著作を一覧する
Nishihara, Hiroshi 著作を一覧する
Kimura, Taichi 著作を一覧する
Kato, Yasutaka 著作を一覧する
Tanino, Mishie 著作を一覧する
Nishio, Mitsufumi 著作を一覧する
Obara, Masato 著作を一覧する
Endo, Tomoyuki 著作を一覧する
Koike, Takao 著作を一覧する
Tanaka, Shinya 著作を一覧する
キーワード: DOCK2
B cell lymphoma
Hematopoietic matignancy
発行日: 2010年 4月23日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 395
号: 1
開始ページ: 111
終了ページ: 115
出版社 DOI: 10.1016/j.bbrc.2010.03.148
抄録: DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 西原 広史


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