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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma
| Title: | DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma |
| Authors: | Wang, Lei Browse this author | | Nishihara, Hiroshi Browse this author →KAKEN DB | | Kimura, Taichi Browse this author | | Kato, Yasutaka Browse this author | | Tanino, Mishie Browse this author | | Nishio, Mitsufumi Browse this author | | Obara, Masato Browse this author | | Endo, Tomoyuki Browse this author | | Koike, Takao Browse this author | | Tanaka, Shinya Browse this author |
| Keywords: | DOCK2 | | B cell lymphoma | | Rac | | ERK | | Hematopoietic matignancy |
| Issue Date: | 23-Apr-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 395 |
| Issue: | 1 |
| Start Page: | 111 |
| End Page: | 115 |
| Publisher DOI: | 10.1016/j.bbrc.2010.03.148 |
| PMID: | 20350533 |
| Abstract: | DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43094 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 西原 広史
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