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Characterization of the interaction of influenza virus NS1 with Akt

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タイトル: Characterization of the interaction of influenza virus NS1 with Akt
著者: Matsuda, Mami 著作を一覧する
Suizu, Futoshi 著作を一覧する
Hirata, Noriyuki 著作を一覧する
Miyazaki, Tadaaki 著作を一覧する
Obuse, Chikashi 著作を一覧する
Noguchi, Masayuki 著作を一覧する
キーワード: Influenza virus
Non-structural protein 1 (NS1)
発行日: 2010年 5月 7日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 395
号: 3
開始ページ: 312
終了ページ: 317
出版社 DOI: 10.1016/j.bbrc.2010.03.166
抄録: Avian influenza viruses belong to the genus influenza A virus of the family Orthomyxoviridae. The influenza virus consists of eight segmented minus stranded RNA that encode 11 known proteins. Among the 11 viral proteins, NS1 (non-structural protein 1, encoded on segment 8) has been implicated in the regulation of several important intra-cellular functions. In this report, we investigated the functional interaction of NS1 with serine threonine kinase Akt, a core intra-cellular survival regulator. In co-immunoprecipitation assays and GST pull-down assays, NS1 directly interacted with Akt. The interaction was mediated primarily through the Akt-PH (Pleckstrin Homology) domain and the RNA-binding domain of NS1. NS1 preferentially interacted with phosphorylated Akt, but not with non-phosphorylated Akt. Functionally, the NS1-Akt interaction enhanced Akt kinase activity both in the intra-cellular context and in in vitro Akt kinase assays. Confocal microscopic analysis revealed that phosphorylated Akt interacted with NS1 during the interphase of the cell cycle predominantly within the nucleus. Finally, mass spectrometric analysis demonstrated the position at Thr215 of NS1 protein is primary phosphorylation target site through Akt activation. The results together supported the functional importance of influenza virus NS1 with Akt, a core intra-cellular survival regulator.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 野口 昌幸


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