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Hokkaido University Collection of Scholarly and Academic Papers >
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Comparison of warfarin sensitivity between rat and bird species
| Title: | Comparison of warfarin sensitivity between rat and bird species |
| Authors: | Watanabe, Kensuke P. Browse this author | | Saengtienchai, Aksorn Browse this author | | Tanaka, Kazuyuki D. Browse this author | | Ikenaka, Yoshinori Browse this author →KAKEN DB | | Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | Avian | | Cytochrome P450 (CYP) | | Inhibitor constant (Ki) | | Raptor | | Vitamin K epoxide reductase (VKOR) | | Vitamin K | | Warfarin |
| Issue Date: | Jun-2010 |
| Publisher: | Elsevier |
| Journal Title: | Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology |
| Volume: | 152 |
| Issue: | 1 |
| Start Page: | 114 |
| End Page: | 119 |
| Publisher DOI: | 10.1016/j.cbpc.2010.03.006 |
| PMID: | 20346414 |
| Abstract: | Scattering coumarin-derivative rodenticides in broad areas have caused primary- and secondary-poisoning incidents in non-target wild birds. In this study, we compared factors determining warfarin sensitivity between bird species and rats based on vitamin K 2,3-epoxide reductase (VKOR) kinetics, VKOR inhibition by warfarin and warfarin metabolism assays. In VKOR characterization, chickens and ostriches showed significantly lower enzymatic efficiencies than rats (one-sixth and one-third, respectively), suggesting bird species depend more on a non-VKOR vitamin K source. On the other hand, the inhibition constants (Ki) of VKOR for warfarin were significantly different between chickens and ostriches (113 ± 2.5 μM and 0.64 ± 0.39 μM, respectively). Interestingly, the ostrich Ki was similar to the values for rats (0.28 ± 0.09 μM). The Ki results reveal a surprising possibility that VKOR in some bird species are easily inhibited by warfarin. Warfarin metabolism assays also showed a large inter-species difference in bird species. Chickens and ostriches showed higher metabolic activity than that of rats, while mallards and owls showed only a slight ability to metabolize warfarin. In this study, we clarified the wide inter-species difference that exists among birds in xenobiotic metabolism and sensitivity to a rodenticide. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43139 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石塚 真由美
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