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Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/43148

Title: Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix
Authors: Ishihara, Seiichiro Browse this author
Haga, Hisashi Browse this author
Yasuda, Motoaki Browse this author →KAKEN DB
Mizutani, Takeomi Browse this author
Kawabata, Kazushige Browse this author
Shirato, Hiroki Browse this author
Nishioka, Takeshi Browse this author
Keywords: Lung adenocarcinoma
Irradiation
3D culture
Collagen gel
Invasion
Integrin β1
Issue Date: 4-Jun-2010
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 396
Issue: 3
Start Page: 651
End Page: 655
Publisher DOI: 10.1016/j.bbrc.2010.04.150
PMID: 20438698
Abstract: Radiotherapy is one of the effective therapies used for treating various malignant tumors. However, the emergence of tolerant cells after irradiation remains problematic due to their high metastatic ability, sometimes indicative of poor prognosis. In this study, we showed that subcloned human lung adenocarcinoma cells (A549P-3) that are irradiation-tolerant indicate high invasive activity in vitro, and exhibit an integrin β1 activity-dependent migratory pattern. In collagen gel overlay assay, majority of the A549P-3 cells displayed round morphology and low migration activity, whereas a considerable number of A549P-3IR cells surviving irradiation displayed a spindle morphology and high migration rate. Blocking integrin β1 activity reduced the migration rate of A549P-3IR cells and altered the cell morphology allowing them to assume a round shape. These results suggest that the A549P-3 cells surviving irradiation acquire a highly invasive integrin β1-dependent phenotype, and integrin β1 might be a potentially effective therapeutic target in combination with radiotherapy.
Type: article (author version)
URI: http://hdl.handle.net/2115/43148
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 芳賀 永

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