|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix
| Title: | Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix |
| Authors: | Ishihara, Seiichiro Browse this author | | Haga, Hisashi Browse this author | | Yasuda, Motoaki Browse this author →KAKEN DB | | Mizutani, Takeomi Browse this author | | Kawabata, Kazushige Browse this author | | Shirato, Hiroki Browse this author | | Nishioka, Takeshi Browse this author |
| Keywords: | Lung adenocarcinoma | | Irradiation | | 3D culture | | Collagen gel | | Invasion | | Integrin β1 |
| Issue Date: | 4-Jun-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 396 |
| Issue: | 3 |
| Start Page: | 651 |
| End Page: | 655 |
| Publisher DOI: | 10.1016/j.bbrc.2010.04.150 |
| PMID: | 20438698 |
| Abstract: | Radiotherapy is one of the effective therapies used for treating various malignant tumors. However, the emergence of tolerant cells after irradiation remains problematic due to their high metastatic ability, sometimes indicative of poor prognosis. In this study, we showed that subcloned human lung adenocarcinoma cells (A549P-3) that are irradiation-tolerant indicate high invasive activity in vitro, and exhibit an integrin β1 activity-dependent migratory pattern. In collagen gel overlay assay, majority of the A549P-3 cells displayed round morphology and low migration activity, whereas a considerable number of A549P-3IR cells surviving irradiation displayed a spindle morphology and high migration rate. Blocking integrin β1 activity reduced the migration rate of A549P-3IR cells and altered the cell morphology allowing them to assume a round shape. These results suggest that the A549P-3 cells surviving irradiation acquire a highly invasive integrin β1-dependent phenotype, and integrin β1 might be a potentially effective therapeutic target in combination with radiotherapy. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43148 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 芳賀 永
|
