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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Mutagenic activation and detoxification of benzo[a]pyrene in vitro by hepatic cytochrome P450 1A1 and phase II enzymes in three meat-producing animals
| Title: | Mutagenic activation and detoxification of benzo[a]pyrene in vitro by hepatic cytochrome P450 1A1 and phase II enzymes in three meat-producing animals |
| Authors: | Darwish, W. Browse this author | | Ikenaka, Y. Browse this author | | Eldaly, E. Browse this author | | Ishizuka, M. Browse this author |
| Keywords: | Mutagenesis | | B[a]P | | CYP1A1 | | Phase II enzymes | | Meat-producing animals |
| Issue Date: | Aug-2010 |
| Publisher: | Elsevier |
| Journal Title: | Food and Chemical Toxicology |
| Volume: | 48 |
| Issue: | 8-9 |
| Start Page: | 2526 |
| End Page: | 2531 |
| Publisher DOI: | 10.1016/j.fct.2010.06.026 |
| PMID: | 20600538 |
| Abstract: | The mutagenic activation activity of hepatic microsomes from three meat-producing animals (cattle, deer and horses) was compared with those of rats as a reference species. In the Ames Salmonella typhimurium TA98 assay, the liver microsomes of all examined animals mutagenically activated benzo[a]pyrene, an ideal promutagens, in terms of production of histidine-independent revertant colonies. The microsomes of horses had the highest ability to produce revertant colonies of the examined animals under both low and high substrate concentrations. Inhibition of this mutagenic activity using α-naphthoflavone, anti-rat CYP1A1, CYP3A2 and CYP2E1 antibodies suggests that this activity was mainly because of CYP1A1 in these animals as well as in rats. The addition of co-factors for two phase II enzymes, microsomal UDP glucoronosyl transferase and cytosolic glutathione-S-transferase, reduced the production of the revertant colonies in a concentration-dependent manner. Interestingly, horses had the highest reduction rate among the examined animals, suggesting that phase II enzymes play a great role in producing a state of balance between the bioactivation and detoxification of xenobiotics in these meat-producing animals. This report is the first to investigate the mutagenic activation activity of the hepatic microsomes and the role of phase II enzymes against this activity in meat-producing animals. |
| Relation: | http://www.sciencedirect.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43284 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石塚 真由美
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