| Title: | Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae. |
| Authors: | Miki, Shinsuke Browse this author |
| Matsui, Kotaro Browse this author |
| Kito, Hideki Browse this author |
| Otsuka, Keisuke Browse this author |
| Ashizawa, Taketo Browse this author |
| Yasuda, Nobuko Browse this author |
| Fukiya, Satoru Browse this author |
| Sato, Junko Browse this author |
| Hirayae, Kazuyuki Browse this author |
| Fujita, Yoshikatsu Browse this author |
| Nakajima, Toshihiko Browse this author |
| Tomita, Fusao Browse this author |
| Sone, Teruo Browse this author →KAKEN DB |
| Keywords: | Gene-for-gene |
| Pyricularia |
| RAPD |
| Issue Date: | May-2009 |
| Publisher: | Blackwell Publishing |
| Journal Title: | Molecular Plant Pathology |
| Volume: | 10 |
| Issue: | 3 |
| Start Page: | 361 |
| End Page: | 374 |
| Publisher DOI: | 10.1111/j.1364-3703.2009.00534.x |
| PMID: | 19400839 |
| Abstract: | In order to clone and analyse the avirulence gene AVR-Pia from Japanese field isolates of Magnaporthe oryzae, a mutant of the M. oryzae strain Ina168 was isolated. This mutant, which was named Ina168m95-1, gained virulence towards the rice cultivar Aichi-asahi, which contains the resistance gene Pia. A DNA fragment (named PM01) that was deleted in the mutant and that co-segregated with avirulence towards Aichi-asahi was isolated. Three cosmid clones that included the regions that flanked PM01 were isolated from a genomic DNA library. One of these clones (46F3) complemented the mutant phenotype, which indicated clearly that this clone contained the avirulence gene AVR-Pia. Clone 46F3 contained insertions of transposable elements. The 46F3 insert was divided into fragments I-VI, and these were cloned individually into a hygromycin-resistant vector for the transformation of the mutant Ina168m95-1. An inoculation assay of the transformants revealed that fragment V (3.5 kb) contained AVR-Pia. By deletion analysis of fragment V, AVR-Pia was localized to an 1199-bp DNA fragment, which included a 255-bp open reading frame with weak homology to a bacterial cytochrome-c-like protein. Restriction fragment length polymorphism analysis of this region revealed that this DNA sequence co-segregated with the AVR-Pia locus in a genetic map that was constructed using Chinese isolates. |
| Rights: | The definitive version is available at www.blackwell-synergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43305 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
