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Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.

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Title: Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.
Authors: Miki, Shinsuke Browse this author
Matsui, Kotaro Browse this author
Kito, Hideki Browse this author
Otsuka, Keisuke Browse this author
Ashizawa, Taketo Browse this author
Yasuda, Nobuko Browse this author
Fukiya, Satoru Browse this author
Sato, Junko Browse this author
Hirayae, Kazuyuki Browse this author
Fujita, Yoshikatsu Browse this author
Nakajima, Toshihiko Browse this author
Tomita, Fusao Browse this author
Sone, Teruo Browse this author →KAKEN DB
Keywords: Gene-for-gene
Issue Date: May-2009
Publisher: Blackwell Publishing
Journal Title: Molecular Plant Pathology
Volume: 10
Issue: 3
Start Page: 361
End Page: 374
Publisher DOI: 10.1111/j.1364-3703.2009.00534.x
PMID: 19400839
Abstract: In order to clone and analyse the avirulence gene AVR-Pia from Japanese field isolates of Magnaporthe oryzae, a mutant of the M. oryzae strain Ina168 was isolated. This mutant, which was named Ina168m95-1, gained virulence towards the rice cultivar Aichi-asahi, which contains the resistance gene Pia. A DNA fragment (named PM01) that was deleted in the mutant and that co-segregated with avirulence towards Aichi-asahi was isolated. Three cosmid clones that included the regions that flanked PM01 were isolated from a genomic DNA library. One of these clones (46F3) complemented the mutant phenotype, which indicated clearly that this clone contained the avirulence gene AVR-Pia. Clone 46F3 contained insertions of transposable elements. The 46F3 insert was divided into fragments I-VI, and these were cloned individually into a hygromycin-resistant vector for the transformation of the mutant Ina168m95-1. An inoculation assay of the transformants revealed that fragment V (3.5 kb) contained AVR-Pia. By deletion analysis of fragment V, AVR-Pia was localized to an 1199-bp DNA fragment, which included a 255-bp open reading frame with weak homology to a bacterial cytochrome-c-like protein. Restriction fragment length polymorphism analysis of this region revealed that this DNA sequence co-segregated with the AVR-Pia locus in a genetic map that was constructed using Chinese isolates.
Rights: The definitive version is available at
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 曾根 輝雄

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