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The covalent modification of spectrin in red cell membranes by the lipid peroxidation product 4-hydroxy-2-nonenal

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Title: The covalent modification of spectrin in red cell membranes by the lipid peroxidation product 4-hydroxy-2-nonenal
Authors: Arashiki, Nobuto Browse this author
Otsuka, Yayoi Browse this author
Ito, Daisuke Browse this author
Yang, Mira Browse this author
Komatsu, Tomohiko Browse this author
Sato, Kota Browse this author →KAKEN DB
Inaba, Mutsumi Browse this author →KAKEN DB
Keywords: spectrin
lipid peroxidation
red cell
Issue Date: 15-Jan-2010
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 391
Issue: 3
Start Page: 1543
End Page: 1547
Publisher DOI: 10.1016/j.bbrc.2009.12.121
PMID: 20036642
Abstract: Spectrin strengthens the red cell membrane through its direct association with membrane lipids and through protein-protein interactions. Spectrin loss reduces the membrane stability and results in various types of hereditary spherocytosis. However, less is known about acquired spectrin damage. Here, we showed that α- and β-spectrin in human red cells are the primary targets of the lipid peroxidation product 4-hydroxy-2-nonenal (HNE) by immunoblotting and mass spectrometry analyses. The level of HNE adducts in spectrin (particularly α-spectrin) and several other membrane proteins was increased following the HNE treatment of red cell membrane ghosts prepared in the absence of MgATP. In contrast, ghost preparation in the presence of MgATP reduced HNE adduct formation, with preferential β-spectrin modification and increased cross-linking of the HNE-modified spectrins. Exposure of intact red cells to HNE resulted in selective HNE-spectrin adduct formation with a similar preponderance of HNE–β-spectrin modifications. These findings indicate that HNE adduction occurs preferentially in spectrin at the interface between the skeletal proteins and lipid bilayer in red cells and suggest that HNE-spectrin adduct aggregation results in the extrusion of damaged spectrin and membrane lipids under physiological and disease conditions.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 稲葉 睦

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