|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
The covalent modification of spectrin in red cell membranes by the lipid peroxidation product 4-hydroxy-2-nonenal
| Title: | The covalent modification of spectrin in red cell membranes by the lipid peroxidation product 4-hydroxy-2-nonenal |
| Authors: | Arashiki, Nobuto Browse this author | | Otsuka, Yayoi Browse this author | | Ito, Daisuke Browse this author | | Yang, Mira Browse this author | | Komatsu, Tomohiko Browse this author | | Sato, Kota Browse this author →KAKEN DB | | Inaba, Mutsumi Browse this author →KAKEN DB |
| Keywords: | spectrin | | 4-hydroxy-2-nonenal | | lipid peroxidation | | red cell | | membrane |
| Issue Date: | 15-Jan-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 391 |
| Issue: | 3 |
| Start Page: | 1543 |
| End Page: | 1547 |
| Publisher DOI: | 10.1016/j.bbrc.2009.12.121 |
| PMID: | 20036642 |
| Abstract: | Spectrin strengthens the red cell membrane through its direct association with membrane lipids and through protein-protein interactions. Spectrin loss reduces the membrane stability and results in various types of hereditary spherocytosis. However, less is known about acquired spectrin damage. Here, we showed that α- and β-spectrin in human red cells are the primary targets of the lipid peroxidation product 4-hydroxy-2-nonenal (HNE) by immunoblotting and mass spectrometry analyses. The level of HNE adducts in spectrin (particularly α-spectrin) and several other membrane proteins was increased following the HNE treatment of red cell membrane ghosts prepared in the absence of MgATP. In contrast, ghost preparation in the presence of MgATP reduced HNE adduct formation, with preferential β-spectrin modification and increased cross-linking of the HNE-modified spectrins. Exposure of intact red cells to HNE resulted in selective HNE-spectrin adduct formation with a similar preponderance of HNE–β-spectrin modifications. These findings indicate that HNE adduction occurs preferentially in spectrin at the interface between the skeletal proteins and lipid bilayer in red cells and suggest that HNE-spectrin adduct aggregation results in the extrusion of damaged spectrin and membrane lipids under physiological and disease conditions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43766 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 稲葉 睦
|
