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Modification of KDM-2 with Culture-spent Medium for Isolation of Renibacterium salmoninarum

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Title: Modification of KDM-2 with Culture-spent Medium for Isolation of Renibacterium salmoninarum
Authors: Matsui, Takanori Browse this author
Nishizawa, Toyohiko Browse this author →KAKEN DB
Yoshimizu, Mamoru Browse this author →KAKEN DB
Keywords: Renibacterium salmoninarum
bacterial kidney disease
growth rate
Issue Date: Sep-2009
Publisher: Japanese Society of Fish Pathology
Journal Title: Fish Pathology
Journal Title(alt): 魚病研究
Volume: 44
Issue: 3
Start Page: 139
End Page: 144
Publisher DOI: 10.3147/jsfp.44.139
Abstract: KDM-2 is a medium widely used for isolation of Renibacterium salmoninarum (R.s.), the causative agent of bacterial kidney disease (BKD). KDM-2 still has a problem for colonization of R.s. at low concentration levels. In the present study, we modified KDM by supplementation of the culture-spent medium of R.s. (SMRs) in substitution of FBS. No difference was observed in the growth rate of R.s. at ≥ 10^[3] cells/mL in KDM broth with 1% FBS regardless of SMRs supplementation. Growth rate of R.s. decreased at 10^[1] cells/mL of inoculation into KDM with 1% FBS, but it was recovered by supplementation of ≥ 1% SMRs into the medium. The activity of SMRs supporting bacterial growth was stable to treatment at 60℃ for 30 min and freezing at -20℃ for 7 days. At inoculation of ≤ 300 CFU of R.s., expected colony counts were obtained on the agar plates containing SMRs, while non or less than half of bacteria colonized on the agar plates without SMRs. It was thus considered that the modified KDM by supplementation of SMRs instead of FBS was convenient and inexpensive for isolation of R.s., especially at low concentration levels.
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 西澤 豊彦

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