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Purification and characterization of a novel incomplete-type vitellogenin protein (VgC) in Sakhalin taimen (Hucho perryi)

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Title: Purification and characterization of a novel incomplete-type vitellogenin protein (VgC) in Sakhalin taimen (Hucho perryi)
Authors: Amano, Haruna Browse this author
Mochizuki, Machiko Browse this author
Fujita, Toshiaki Browse this author
Hiramatsu, Naoshi Browse this author
Todo, Takashi Browse this author →KAKEN DB
Hara, Akihiko Browse this author →KAKEN DB
Keywords: Multiple vitellogenin
Purification
Quantification
Sakhalin taimen
Issue Date: Sep-2010
Publisher: Elsevier
Journal Title: Comparative Biochemistry and Physiology, Part A : Molecular & Integrative Physiology
Volume: 157
Issue: 1
Start Page: 41
End Page: 48
Publisher DOI: 10.1016/j.cbpa.2010.05.006
PMID: 20472090
Abstract: A novel, incomplete-type vitellogenin (VgC) and its derived yolk lipovitellin (LvC) were immunologically detected in female serum and egg extracts, respectively, of Sakhalin taimen (Hucho perryi) using a subtype-specific antiserum against LvC of grey mullet (Mugil cephalus). The taimen VgC was purified from the sera of vitellogenic females by a combination of gel filtration, anion exchange, and immunoadsorbent column chromatography. Gel filtration of the purified VgC revealed that it had an apparent native mass of ∼380 kDa, while the mass of the VgC polypeptide that appeared following SDS-PAGE was estimated to be ∼140 kDa. An antiserum was raised against the purified VgC and utilized for the development of a subtype-specific immunoassay for VgC. Levels of VgC in the serum of female taimen increased from 25 μg/mL to ∼1 mg/mL, with an increase of GSI. Levels of complete-type Vg and estradiol-17β (E2) in the serum of E2-administered juvenile taimen increased and reached peak levels similar to those found in vitellogenic females. Although VgC could be induced in the serum of E2-administered taimen, it stayed at levels (35.5-73 μg/mL) lower than those obtained in females. This is the first report on the presence of serum VgC and yolk LvC in a salmonid species; these findings indicate that for Sakhalin taimen, like other highly-evolved teleost species, this minor subtype of Vg is significant in the formation of egg yolk.
Type: article (author version)
URI: http://hdl.handle.net/2115/43820
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 原 彰彦

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