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An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai

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Title: An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai
Authors: Zahura, Umme Afsari Browse this author
Rahman, Mohammad Matiur Browse this author
Inoue, Akira Browse this author →KAKEN DB
Tanaka, Hiroyuki Browse this author →KAKEN DB
Ojima, Takao Browse this author →KAKEN DB
Keywords: Sea hare
Mollusks
Gastropod
β-1,4-mannanase
Mannan
Mannooligosaccharide
Locust bean gum
Issue Date: Sep-2010
Publisher: Elsevier
Journal Title: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Volume: 157
Issue: 1
Start Page: 137
End Page: 143
Publisher DOI: 10.1016/j.cbpb.2010.05.012
PMID: 20639136
Abstract: A mannan-degrading enzyme was isolated from the digestive fluid of the common sea hare Aplysia kurodai by ammonium sulfate fractionation followed by conventional column chromatography. The purified enzyme, named AkMan in the present paper, showed a single band with an approximate molecular mass of 40,000 Da on SDS-PAGE and preferably degraded a linear β-1,4-mannan from green algae Codium fragile producing triand disaccharides. The optimal temperature of AkMan was 55 °C at pH 7.0 and temperature that caused 50% inactivation of AkMan during a 20-min incubation was 52 °C. AkMan retained high activity at pH 4.0–7.5 and was not inactivated in such acidic pH range by the incubation at 40 °C for 20 min. AkMan could degrade glucomannan from konjak root and galactomannan (tara gum and guar gum) as well as the linear β-1,4- mannan, while not carboxymethyl cellulose, agarose, dextran and xylan. These results indicate that AkMan is a typical endo-β-1,4-mannanase (EC 3.2.1.78) splitting internal β-1,4-mannosyl linkages of mannan. The Nterminal and internal amino-acid sequences of AkMan shared ∼55% amino-acid identity to the corresponding sequences of an abalone β-1,4-mannanase, HdMan, which belongs to glycosyl hydrolase family 5 (GHF5). Thus, AkMan was also regarded as a member of GHF5 β-1,4-mannanases.
Relation: http://www.elsevier.com/locate/cbpb
Type: article (author version)
URI: http://hdl.handle.net/2115/43831
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 尾島 孝男

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