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Title: 有害化学物質吸着のための二重らせん構造を保持したDNA/ポリウレタンフォーム複合材料の開発
Other Titles: Development of DNA/Polyurethane Composite Foam for Adsorption of Chemical Hazards
Authors: 曾根, 弘昭1 Browse this author
古月, 文志2 Browse this author →KAKEN DB
田中, 俊逸3 Browse this author →KAKEN DB
Authors(alt): Sone, Hiroaki1
Fugetsu, Bunshi2
Tanaka, Shunitz3
Keywords: double-helix DNA
polyurethane foam
Issue Date: 5-Jan-2009
Publisher: 日本分析化学会
Journal Title: 分析化学
Volume: 58
Issue: 1
Start Page: 7
End Page: 13
Publisher DOI: 10.2116/bunsekikagaku.58.7
Abstract: 特異的な構造を有する二重らせんDNAは,主にインターカレーションと呼ばれる作用によって平面構造を有する有害化学物質を捕捉することが知られている.しかし,機械的強度の脆弱性や高い水溶性等の問題点があり,吸着材としての利用は長らく限定されていた.本研究において,DNA/ポリウレタン複合発泡材料(DNA/PUCF)を作製することによって,二重らせんDNAをポリウレタンフォームに封入し,それらの問題点を解決した.作製したDNA/PUCF の吸着性能評価は,代表的な平面構造を有する有害化学物質(臭化エチジウム,アクリジンオレンジ及びエオシンB)を用いて行われた.その結果,DNA/PUCFは陽イオン性である臭化エチジウム及びアクリジンオレンジに対して高い吸着能を示したが,陰イオン性であるエオシンBに対しては吸着能を示さなかった.この実験結果より,DNA塩基対へのインターカレーションには,有害物質の持つ電荷が大きく寄与することが示された.更に,金属イオンの吸着実験を行ったところ,DNA/PUCFへの吸着は,銀イオンは他金属イオンよりも親和性が高い結果を得た.DNA/PUCFを作製することによって,伸縮性があり,機械的強度が強く,耐久性及び利便性の高い,平面構造を有する陽イオン性有害化学物質除去材が得られた.
Double-helix DNA, because of its unique morphology, is capable for capturing chemical hazards of planer structures, mainly due to the so-called intercalation interactions. Its intrinsic properties, such as mechanical weakness and high water solubility, however, have long been drawbacks restricting practical applications. These intrinsic difficulties of DNA were overcome in this study by encapsulation of the double-helix DNA using polyurethane by the formation of DNA/polyurethane composite foams (DNA/PUCF). The adsorptive capability of the resulting DNA/PUCF was evaluated using ethidium bromide, acridine orange and eosin B, as typically hazardous chemicals having planer structures. DNA/PUCF has shown high ability for absorbing ethidium bromide and acridine orange, both carrying positively charged moieties ; but no ability was found for eosin B, which is a negatively charged species, indicating that the charges on the hazardous molecules play an important rule in determining their distribution into the base-pairs of DNA where the intercalation takes a place. The investigation of DNA by using silver ions (Ag+) as reactive ions gave experimental evidence to confirm a fact that DNA involved in the DNA/PUCF kept their double-helix structures. DNA/PUCF is robust, mechanically strong, durable, and highly useful for eliminating positively charged, water-soluble type of hazardous chemicals of planer structures.
Type: article
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 古月 文志

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