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Eicosapentaenoic acid suppresses ocular inflammation in endotoxin-induced uveitis

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Title: Eicosapentaenoic acid suppresses ocular inflammation in endotoxin-induced uveitis
Authors: Suzuki, Misa Browse this author
Noda, Kousuke Browse this author
Kubota, Shunsuke Browse this author
Hirasawa, Manabu Browse this author
Ozawa, Yoko Browse this author
Tsubota, Kazuo Browse this author
Mizuki, Nobuhisa Browse this author
Ishida, Susumu Browse this author
Issue Date: 17-Jul-2010
Publisher: Molecular Vision
Journal Title: Molecular Vision
Volume: 16
Start Page: 1382
End Page: 1388
Abstract: Purpose: To investigate the effect of eicosapentaenoic acid (EPA) on acute ocular inflammation in an animal model of endotoxin-induced uveitis (EIU). Methods: C57Bl/6 mice (6-week-old males) were orally treated with EPA at a dose of 50 mg/kg/day for 5 days. EIU was then induced in the animals by intraperitoneal injection of 160 μg lipopolysaccharide (LPS). Twenty-four hours after LPS injection, leukocyte adhesion to the retinal vasculature was evaluated by the concanavalin A lectin perfusion-labeling technique, and leukocyte infiltration into the vitreous cavity was quantified. Furthermore, the protein levels of monocyte chemotactic protein (MCP)-1, interleukin (IL)-6, intercellular adhesion molecule-1 and phospholyrated nuclear factor (NF)-κB p65 in the retina and retinal pigment epithelium (RPE)-choroid complex were examined by enzyme-linked immunosorbent assay (ELISA). Results: At 24 h after LPS injection, the EIU animals treated with oral EPA administration showed a significant decrease in leukocyte adhesion to the retinal vessels by 43.4% (p< 0.01) and leukocyte infiltration into the vitreous cavity by 49.2% (p<0.05). In addition, EPA significantly reduced the protein levels of MCP-1 and IL-6 in the retina and the RPE-choroid complex. Furthermore, phosphorylation of NF-κB was suppressed by EPA treatment. Conclusions: Our data suggest that EPA inhibits multiple inflammatory molecules in vivo. EPA may become a novel strategy in the prevention and/or treatment of ocular inflammatory diseases.
Type: article
URI: http://hdl.handle.net/2115/43846
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石田 晋

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