|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates
| Title: | Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates |
| Authors: | Matsuo, Junji Browse this author →KAKEN DB | | Kawaguchi, Kouhei Browse this author | | Nakamura, Shinji Browse this author →KAKEN DB | | Hayashi, Yasuhiro Browse this author →KAKEN DB | | Yoshida, Mitsutaka Browse this author | | Takahashi, Kaori Browse this author | | Mizutani, Yoshihiko Browse this author | | Yao, Takashi Browse this author | | Yamaguchi, Hiroyuki Browse this author →KAKEN DB |
| Issue Date: | Aug-2010 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Environmental Microbiology Reports |
| Volume: | 2 |
| Issue: | 4 |
| Start Page: | 524 |
| End Page: | 533 |
| Publisher DOI: | 10.1111/j.1758-2229.2009.00094.x |
| PMID: | 23766223 |
| Abstract: | Obligate intracellular bacteria are commonly found as endosymbionts of acanthamoebae, however, their survival in and ability to transfer to amoebae are currently uncharacterized. In this study, six bacterial endosymbionts, found in five environmental Acanthamoeba isolates (S13, R18, S23, S31, S40) from different locations of Sapporo city, Japan, were characterized. Phylogenetic analysis revealed that three-bacterial endosymbionts (eS31, eS40a, eS23) belonged to α- and β-Proteobacterium phyla and the remaining endosymbionts (eR18, eS13, eS40b) belonged to the Chlamydiales phylum. The Acanthamoeba isolate (S40) contained two phylogenetically different bacterial endosymbionts (eS40a, eS40b). Fluorescent in situ hybridization analysis showed that all bacterial endosymbionts were diffusely localized within amoebae. Transmission electron microscopy also showed that the endosymbionts were rod-shaped (eS31, eS40a, eS23) or sphere- or crescent-shaped (eR18, eS13, eS40b). No successful culture of these bacteria was achieved using conventional culture methods, but the viability of endosymbionts was confirmed by live/dead staining and RT-PCR methods. However, endosymbionts (except eR18) derived from original host cells lost the ability to be transferred to another amoeba strain (Acanthamoebae ATCC C3). Taken together, our data demonstrate that phylogenetically diverse bacterial endosymbionts found in amoebae are viable and maintain a stable interaction with amoebae. |
| Rights: | This is the pre-peer reviewed version of the following
article: Environmental Microbiology Reports, 2(4), 524-533, Aug. 2010, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/j.1758-2229.2009.00094.x/abstract |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43886 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 山口 博之
|
