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Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates

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Title: Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates
Authors: Matsuo, Junji Browse this author →KAKEN DB
Kawaguchi, Kouhei Browse this author
Nakamura, Shinji Browse this author →KAKEN DB
Hayashi, Yasuhiro Browse this author →KAKEN DB
Yoshida, Mitsutaka Browse this author
Takahashi, Kaori Browse this author
Mizutani, Yoshihiko Browse this author
Yao, Takashi Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Issue Date: Aug-2010
Publisher: Wiley-Blackwell
Journal Title: Environmental Microbiology Reports
Volume: 2
Issue: 4
Start Page: 524
End Page: 533
Publisher DOI: 10.1111/j.1758-2229.2009.00094.x
PMID: 23766223
Abstract: Obligate intracellular bacteria are commonly found as endosymbionts of acanthamoebae, however, their survival in and ability to transfer to amoebae are currently uncharacterized. In this study, six bacterial endosymbionts, found in five environmental Acanthamoeba isolates (S13, R18, S23, S31, S40) from different locations of Sapporo city, Japan, were characterized. Phylogenetic analysis revealed that three-bacterial endosymbionts (eS31, eS40a, eS23) belonged to α- and β-Proteobacterium phyla and the remaining endosymbionts (eR18, eS13, eS40b) belonged to the Chlamydiales phylum. The Acanthamoeba isolate (S40) contained two phylogenetically different bacterial endosymbionts (eS40a, eS40b). Fluorescent in situ hybridization analysis showed that all bacterial endosymbionts were diffusely localized within amoebae. Transmission electron microscopy also showed that the endosymbionts were rod-shaped (eS31, eS40a, eS23) or sphere- or crescent-shaped (eR18, eS13, eS40b). No successful culture of these bacteria was achieved using conventional culture methods, but the viability of endosymbionts was confirmed by live/dead staining and RT-PCR methods. However, endosymbionts (except eR18) derived from original host cells lost the ability to be transferred to another amoeba strain (Acanthamoebae ATCC C3). Taken together, our data demonstrate that phylogenetically diverse bacterial endosymbionts found in amoebae are viable and maintain a stable interaction with amoebae.
Rights: This is the pre-peer reviewed version of the following article: Environmental Microbiology Reports, 2(4), 524-533, Aug. 2010, which has been published in final form at
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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