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Functional analysis of an α-helical antimicrobial peptide derived from a novel mouse defensin-like gene
| Title: | Functional analysis of an α-helical antimicrobial peptide derived from a novel mouse defensin-like gene |
| Authors: | Kawaguchi, Akira Browse this author | | Suzuki, Tadaki Browse this author | | Kimura, Takashi Browse this author | | Sakai, Naoki Browse this author | | Ayabe, Tokiyoshi Browse this author | | Sawa, Hirofumi Browse this author | | Hasegawa, Hideki Browse this author |
| Keywords: | Antimicrobial peptide | | Cationic α-helical peptide | | Defensin | | Bactericidal activity | | CD spectroscopy |
| Issue Date: | 6-Aug-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 398 |
| Issue: | 4 |
| Start Page: | 778 |
| End Page: | 784 |
| Publisher DOI: | 10.1016/j.bbrc.2010.07.028 |
| PMID: | 20637182 |
| Abstract: | Gene-encoded antimicrobial peptides (AMPs) are an essential component of the innate immune system in many species. Analysis of β-defensin gene expression in mouse tissue using primers that were specific for conserved sequences located outside of the β-defensin translated region identified a novel small gene. The novel gene had an open reading frame of 114 basepairs and encoded a predicted protein of 37 amino acid residues. A search of the genome database revealed that the gene locus and the sequence of exon 1 of this novel gene were similar to subgroup 1 mouse β-defensins. A small peptide, K17 (FSPQMLQDIIEKKTKIL), derived from the amino acid sequence of this novel gene was synthesized. Circular dichroism (CD) spectroscopic analysis of chemically synthesized peptide demonstrated that the peptide exhibited random coil conformation in aqueous solution, but the peptide adopted helical conformation in the presence of trifluoroethanol or sodium dodecyl sulfate, a membrane mimicking environment. The peptide exhibited bactericidal activity against Salmonella enterica serovar Typhimurium (Gram negative) and Staphylococcus aureus (Gram positive); it was not cytotoxic in cultures of mammalian cells or hemolytic in cultures of erythrocytes. These results suggested that K17 may be a candidate therapeutic for the treatment of bacterial infection. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43910 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 川口 晶
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