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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
The TLR2 ligand FSL-1 is internalized via clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2
| Title: | The TLR2 ligand FSL-1 is internalized via clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2 |
| Authors: | Haque, Shamsul Browse this author | | Hasebe, Akira Browse this author →KAKEN DB | | Iyori, Mitsuhiro Browse this author | | Ohtani, Makoto Browse this author | | Kiura, Kazuto Browse this author | | Zhang, Diya Browse this author | | Totsuka, Yasunori Browse this author | | Shibata, Ken-ichiro Browse this author |
| Keywords: | TLR2 | | clathrin-dependent endocytosis | | CD14 | | CD36 |
| Issue Date: | 1-Jun-2010 |
| Publisher: | Blackwell Scientific Publications |
| Journal Title: | Immunology : an official journal of the British Society for Immunology |
| Volume: | 130(2) |
| Start Page: | 262 |
| End Page: | 272 |
| Publisher DOI: | 10.1111/j.1365-2567.2009.03232.x |
| PMID: | 20113368 |
| Abstract: | Little is known about how Toll-like receptor (TLR) ligands are processed after recognition by TLRs. This study was therefore designed to investigate how the TLR2 ligand FSL-1 is processed in macrophages after recognition by TLR2. FSL-1 was internalized into murine macrophage cell line, RAW264.7. Both chlorpromazine and methyl-β-cyclodextrin, which inhibit clathrin-dependent endocytosis, reduced FSL-1 uptake by RAW264.7 cells in a dose-dependent manner, but nystatin which inhibits caveolae- and lipid raft-dependent endocytosis, did not. FSL-1 was colocalized with clathrin but not with TLR2 in the cytosol of RAW264.7 cells. These results suggest that internalization of FSL-1 is clathrin dependent. In addition, FSL-1 was internalized by peritoneal macrophages from TLR2-deficient mice. FSL-1 was internalized by human embryonic kidney 293 cells transfected with CD14 or CD36 but not by the non-transfected cells. Also, knockdown of CD14 or CD36 in the transfectants reduced FSL-1 uptake In this study, we suggest that (i) FSL-1 is internalized into macrophages via a clathrin-dependent endocytic pathway, (ii) the FSL-1 uptake by macrophages occurs irrespective of the presence of TLR2, and (iii) CD14 and CD36 are responsible for the 18 internalization of FSL-1. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43949 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 柴田 健一郎
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