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A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli
Title: | A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli |
Authors: | Iyori, Mitsuhiro Browse this author | Ohtani, Makoto Browse this author | Hasebe, Akira Browse this author | Totsuka, Yasunori Browse this author | Shibata, Ken-ichiro Browse this author →KAKEN DB |
Issue Date: | 10-Oct-2008 |
Publisher: | Academic Press |
Journal Title: | Biochemical and biophysical research communications |
Volume: | 377(2) |
Start Page: | 367 |
End Page: | 372 |
PMID: | 18848918 |
Abstract: | HEK293 cells stably expressing DC-SIGN (293/DC-SIGN) were examined for phagocytosis of Escherichia coli. 293/DC-SIGN stable transfectants were able to mediate phagocytosis of E. coli. The phagocytosis was inhibited by EDTA or several inhibitors specific for Syk kinase, Raf kinase and the transcription factor NF-B.DC-SIGN consists of characteristic domains and motifs such as CRD, neck, incomplete ITAM, dileucine and tri-acidic cluster. HEK293 cells expressing mutants of DC-SIGN were also examined for the phagocytosis. It was found that Ca2+ binding sites in the CRD of DC-SIGN were involved in phagocytosis of bacteria as well as multimerization of DC-SIGN, and the neck region played a role in efficiency of binding to microbes as well as multimerization of the protein. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/43954 |
Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 柴田 健一郎
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