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The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/43974

Title: The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses
Authors: Kiura, Kazuto Browse this author
Kataoka, Hideo Browse this author
Yasuda, Motoaki Browse this author
Inoue, Nobuo Browse this author
Shibata, Ken-ichiro Browse this author →KAKEN DB
Keywords: adjuvant
Th2 response
Toll-like receptor 2
lipopeptide
Issue Date: Oct-2006
Publisher: 55
Journal Title: FEMS immunology and medical microbiology
Volume: 48(1)
Start Page: 44
End Page: 55
Publisher DOI: 10.1111/j.1574-695X.2006.00119.x
PMID: 16965351
Abstract: The diacylated lipopeptide FSL-1 enhanced the generation of IgG antibodies in TLR2+/+ mice, but not in TLR2-/- mice, when administered together with hen egg lysozyme as an antigen. Escherichia coli lipopolysaccharide enhanced the generation of antigen-specific antibodies in both TLR2-/- and TLR2+/+ mice. In TLR2+/+ mice, the enhancement level by FSL-1 was similar to that by lipopolysaccharide. Analysis of the IgG antibodies subclass demonstrated that level of Th2-type IgG1 antibodies was higher than that of Th1-type IgG2a antibodies. Both FSL-1 and lipopolysaccharide induced production of IL-10 and IL-6 by splenocytes from TLR2+/+ mice. Lipopolysaccharide also induced production of these cytokines by splenocytes from TLR2-/- mice, but FSL-1 did not. Neither FSL-1 nor lipopolysaccharide induced IL-12p70 production by splenocytes from both types of mice. FSL-1 upregulated B7.2 expression in B220+ cells from TLR2+/+ mice but not those from TLR2-/- mice, whereas lipopolysaccharide upregulated B7.2 expression in B220+ cells from both types of mice. FSL-1 and, to a lesser extent, lipopolysaccharide activated mitogen-activated protein kinases in splenocytes. FSL-1 and, to a lesser extent,lipopolysaccharide induced the expression of c-Fos, which is known to be involved in Th2-type responses, in splenocytes. Thus, this study demonstrated that FSL-1 possessed TLR2-mediated Th2-type responses in vivo.
Type: article (author version)
URI: http://hdl.handle.net/2115/43974
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柴田 健一郎

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