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The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses

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タイトル: The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses
著者: Kiura, Kazuto 著作を一覧する
Kataoka, Hideo 著作を一覧する
Yasuda, Motoaki 著作を一覧する
Inoue, Nobuo 著作を一覧する
Shibata, Ken-ichiro 著作を一覧する
キーワード: adjuvant
Th2 response
Toll-like receptor 2
発行日: 2006年10月
出版者: 55
誌名: FEMS immunology and medical microbiology
巻: 48(1)
開始ページ: 44
終了ページ: 55
出版社 DOI: 10.1111/j.1574-695X.2006.00119.x
抄録: The diacylated lipopeptide FSL-1 enhanced the generation of IgG antibodies in TLR2+/+ mice, but not in TLR2-/- mice, when administered together with hen egg lysozyme as an antigen. Escherichia coli lipopolysaccharide enhanced the generation of antigen-specific antibodies in both TLR2-/- and TLR2+/+ mice. In TLR2+/+ mice, the enhancement level by FSL-1 was similar to that by lipopolysaccharide. Analysis of the IgG antibodies subclass demonstrated that level of Th2-type IgG1 antibodies was higher than that of Th1-type IgG2a antibodies. Both FSL-1 and lipopolysaccharide induced production of IL-10 and IL-6 by splenocytes from TLR2+/+ mice. Lipopolysaccharide also induced production of these cytokines by splenocytes from TLR2-/- mice, but FSL-1 did not. Neither FSL-1 nor lipopolysaccharide induced IL-12p70 production by splenocytes from both types of mice. FSL-1 upregulated B7.2 expression in B220+ cells from TLR2+/+ mice but not those from TLR2-/- mice, whereas lipopolysaccharide upregulated B7.2 expression in B220+ cells from both types of mice. FSL-1 and, to a lesser extent, lipopolysaccharide activated mitogen-activated protein kinases in splenocytes. FSL-1 and, to a lesser extent,lipopolysaccharide induced the expression of c-Fos, which is known to be involved in Th2-type responses, in splenocytes. Thus, this study demonstrated that FSL-1 possessed TLR2-mediated Th2-type responses in vivo.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 柴田 健一郎


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