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Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages

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Title: Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages
Authors: INTO, TAKESHI Browse this author
FUJITA, MARI Browse this author
OKUSAWA, TSUGUMI Browse this author
HASEBE, AKIRA Browse this author
MORITA, MANABU Browse this author
SHIBATA, KEN-ICHIRO Browse this author →KAKEN DB
Issue Date: Jul-2002
Publisher: American Society For Microbiology
Journal Title: Infection and immunity
Volume: 70(7)
Start Page: 3586
End Page: 3591
Publisher DOI: 10.1128/IAI.70.7.3586-3591.2002
PMID: 12065499
Abstract: Mycoplasmal lipopeptides, S-(2,3-bispalmitoyloxypropyl)-CGDPKHSPKSF and S-(2,3-bispalmitoyloxypropyl)-CGNNDESNISFKEK activated a monocytic cell line,THP-1 cells, to produce tumor necrosis factor-α. The activity of the lipopeptides was augmented by ATP added in a dose-dependent manner. In addition, the level of the expression of mRNAs of tumor necrosis factor-α, interleukin-1β, -6 and -8 was also up-regulated by the lipopeptides and/or extracellular ATP, but that of interleukin-10 was not. The P2X purinergic receptor antagonists pyridoxal phosphate 6-azophenyl 2’,4’-disulfonic acid and periodate oxidized ATP suppressed the activity of ATP to augment the activation of THP-1 cells by the lipopeptides, suggesting that P2X receptors play important roles in the activity of ATP. The nuclear factor-κB inhibitor dexamethasone also suppressed the activity,suggesting that the activity of ATP is dependent upon the nuclear factor-κB.Thus, these results suggest that the interaction of extracellular ATP with the P2X receptors is attributed to the activity of ATP to augment the activation of THP-1 cells by mycoplasmal lipopeptides.
Type: article (author version)
URI: http://hdl.handle.net/2115/43983
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柴田 健一郎

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