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Hokkaido University Collection of Scholarly and Academic Papers >
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Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages
| Title: | Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages |
| Authors: | INTO, TAKESHI Browse this author | | FUJITA, MARI Browse this author | | OKUSAWA, TSUGUMI Browse this author | | HASEBE, AKIRA Browse this author | | MORITA, MANABU Browse this author | | SHIBATA, KEN-ICHIRO Browse this author →KAKEN DB |
| Issue Date: | Jul-2002 |
| Publisher: | American Society For Microbiology |
| Journal Title: | Infection and immunity |
| Volume: | 70(7) |
| Start Page: | 3586 |
| End Page: | 3591 |
| Publisher DOI: | 10.1128/IAI.70.7.3586-3591.2002 |
| PMID: | 12065499 |
| Abstract: | Mycoplasmal lipopeptides, S-(2,3-bispalmitoyloxypropyl)-CGDPKHSPKSF and S-(2,3-bispalmitoyloxypropyl)-CGNNDESNISFKEK activated a monocytic cell line,THP-1 cells, to produce tumor necrosis factor-α. The activity of the lipopeptides was augmented by ATP added in a dose-dependent manner. In addition, the level of the expression of mRNAs of tumor necrosis factor-α, interleukin-1β, -6 and -8 was also up-regulated by the lipopeptides and/or extracellular ATP, but that of interleukin-10 was not. The P2X purinergic receptor antagonists pyridoxal phosphate 6-azophenyl 2’,4’-disulfonic acid and periodate oxidized ATP suppressed the activity of ATP to augment the activation of THP-1 cells by the lipopeptides, suggesting that P2X receptors play important roles in the activity of ATP. The nuclear factor-κB inhibitor dexamethasone also suppressed the activity,suggesting that the activity of ATP is dependent upon the nuclear factor-κB.Thus, these results suggest that the interaction of extracellular ATP with the P2X receptors is attributed to the activity of ATP to augment the activation of THP-1 cells by mycoplasmal lipopeptides. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43983 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 柴田 健一郎
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