HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
歯学研究院・歯学院  >
雑誌発表論文等  >

Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

フルテキスト
cell.pdf1.68 MBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/43991

タイトル: Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth
著者: Domon, Takanori 著作を一覧する
Taniguchi, Yumi 著作を一覧する
Inoue, Kiichiro 著作を一覧する
Ushijima, Natsumi 著作を一覧する
Taishi, Yoshihito 著作を一覧する
Hiramatsu, Akiko 著作を一覧する
Wakita, Minoru 著作を一覧する
Yoshida, Shigemitsu 著作を一覧する
キーワード: Odontoclast
Apoptosis
TUNEL method
Transmission electron microscopy
Human
発行日: 2008年
出版者: Springer
誌名: Cell and Tissue Research
巻: 331
号: 2
開始ページ: 423
終了ページ: 433
出版社 DOI: 10.1007/s00441-007-0525-0
抄録: This study was designed to establish the apoptosis of odontoclasts during physiological root resorption of human deciduous teeth. Deciduous teeth were fixed, decalcified, and embedded in paraffin for immunohistochemical (IHC) observations and in Epon for transmission electron microscopy (TEM). Apoptotic cells were identified by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL), and then tartrate-resistant acid phosphatase (TRAP) activity was determined on the same sections. Epon-embedded specimens were sectioned serially into 0.5-μm semithin sections; some of these sections were re-embedded in Epon, sectioned into 0.1-μm ultrathin sections, and observed by TEM. IHC revealed that the nuclei of TRAP-positive odontoclasts on the dentine were generally TUNEL-negative. Around these odontoclasts, a few TRAP-positive structures were present together with TUNEL-positive structures, e.g., a TRAPpositive structure with one TUNEL-positive nucleus, a TRAP-positive structure with one TUNEL-positive nucleus plus one or two TUNEL-negative nuclei, or a TRAP-positive structure with no nucleus. By TEM, some odontoclasts showed nuclear fragments including compacted chromatin. The results suggest that, during apoptosis, odontoclasts fragment into variously sized cellular parts including three or fewer nuclei.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/43991
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 土門 卓文

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学