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Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

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Title: Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth
Authors: Domon, Takanori Browse this author →KAKEN DB
Taniguchi, Yumi Browse this author
Inoue, Kiichiro Browse this author
Ushijima, Natsumi Browse this author
Taishi, Yoshihito Browse this author
Hiramatsu, Akiko Browse this author
Wakita, Minoru Browse this author
Yoshida, Shigemitsu Browse this author
Keywords: Odontoclast
TUNEL method
Transmission electron microscopy
Issue Date: 2008
Publisher: Springer
Journal Title: Cell and Tissue Research
Volume: 331
Issue: 2
Start Page: 423
End Page: 433
Publisher DOI: 10.1007/s00441-007-0525-0
PMID: 18000684
Abstract: This study was designed to establish the apoptosis of odontoclasts during physiological root resorption of human deciduous teeth. Deciduous teeth were fixed, decalcified, and embedded in paraffin for immunohistochemical (IHC) observations and in Epon for transmission electron microscopy (TEM). Apoptotic cells were identified by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL), and then tartrate-resistant acid phosphatase (TRAP) activity was determined on the same sections. Epon-embedded specimens were sectioned serially into 0.5-μm semithin sections; some of these sections were re-embedded in Epon, sectioned into 0.1-μm ultrathin sections, and observed by TEM. IHC revealed that the nuclei of TRAP-positive odontoclasts on the dentine were generally TUNEL-negative. Around these odontoclasts, a few TRAP-positive structures were present together with TUNEL-positive structures, e.g., a TRAPpositive structure with one TUNEL-positive nucleus, a TRAP-positive structure with one TUNEL-positive nucleus plus one or two TUNEL-negative nuclei, or a TRAP-positive structure with no nucleus. By TEM, some odontoclasts showed nuclear fragments including compacted chromatin. The results suggest that, during apoptosis, odontoclasts fragment into variously sized cellular parts including three or fewer nuclei.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 土門 卓文

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