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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Suppression of thermal denaturation of myosin and salt-induced denaturation of actin by sodium citrate in carp (Cyprinus carpio)
| Title: | Suppression of thermal denaturation of myosin and salt-induced denaturation of actin by sodium citrate in carp (Cyprinus carpio) |
| Authors: | Kuwahara, Koichi Browse this author | | Konno, Kunihiko Browse this author →KAKEN DB |
| Keywords: | Sodium citrate (Na-citrate) | | Myosin | | Actin | | Denaturation | | Sorbitol |
| Issue Date: | 15-Oct-2010 |
| Publisher: | Elsevier |
| Journal Title: | Food Chemistry |
| Volume: | 122 |
| Issue: | 4 |
| Start Page: | 997 |
| End Page: | 1002 |
| Publisher DOI: | 10.1016/j.foodchem.2010.03.056 |
| Abstract: | The effect of sodium citrate (Na-citrate) on myosin and actin denaturation in myofibrils was investigated. Na-citrate significantly suppressed the thermal inactivation of Ca2+-ATPase of carp myosin in a concentration dependent manner. The effect was greater than that of sorbitol. A similar effect was observed with myofibrils in which myosin is stabilized by F-actin binding. Na-citrate dissolved myofibrils at lower concentration than NaCl. Nevertheless, Na-citrate at 1 M never denatured F-actin in myofibrils, while 1 M NaCl denatured F-actin almost completely. Na-citrate suppressed the NaCl-induced F-actin denaturation. Sorbitol did not show such protective effect on F-actin denaturation. Moreover, Na-citrate suppressed the freeze denaturation of myofibrils at lower concentration than sorbitol. Thus, Na-citrate was proved to be superior to sorbitol. It was suggested that Na-citrate alone could substitute sorbitol as cryoprotectant in surimi and NaCl as dissolving reagent of myofibril in thermal gel production. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44026 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 今野 久仁彦
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