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Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement

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Title: Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement
Authors: Ikeda, Kensuke Browse this author
Mizutani, Takeomi Browse this author
Hoshi, Osamu Browse this author
Ushiki, Tatsuo Browse this author
Haga, Hisashi Browse this author
Kawabata, Kazushige Browse this author →KAKEN DB
Keywords: Chromosome
Scanning probe microscopy
Intermediate structure
Chromatin fiber
Issue Date: 10-Sep-2010
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 400
Issue: 1
Start Page: 181
End Page: 186
Publisher DOI: 10.1016/j.bbrc.2010.08.041
PMID: 20719238
Abstract: The morphology of chromosomes (certain rod-shaped structures) is highly reproducible despite the high condensation of chromatin fibers (∼1 mm) into chromosomes (∼1 μm). However, the mechanism underlying the condensation of chromatin fibers into chromosomes is unclear. We assume that investigation of the internal structure of chromosomes will aid in elucidating the condensation process. In order to observe the detailed structure of a chromosome, we stretched a human chromosome by using a micromanipulator and observed its morphology along the stretched region by scanning probe microscopy (SPM). We found that the chromosome consisted of some fibers that were thicker than chromatin fibers. The found fiber was composed of approximately 90-nm-wide beads that were linked linearly. To explore the components of the fiber, we performed immunofluorescence staining of the stretched chromosome. Fluorescence signals of topoisomerase (Topo) IIα, which is known to interact with and support chromatin fibers, and DNA were detected both on the found fiber and beads. Furthermore, after micrococcal nuclease and trypsin treatments, the fibers were found to be mechanically supported by proteins. These results suggest that chromosome comprises an intermediate structure between chromatin fibers and chromosomes.
Type: article (author version)
URI: http://hdl.handle.net/2115/44031
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 水谷 武臣

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