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Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/44121

Title: Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis
Authors: Takahashi, Megumu Browse this author
Uji, Toshiki Browse this author →KAKEN DB
Saga, Naotsune Browse this author →KAKEN DB
Mikami, Koji Browse this author →KAKEN DB
Keywords: allantoin
protoplast
P. yezoensis
regeneration
transient gene expression
Issue Date: 15-Mar-2010
Publisher: Pontificia Universidad Católica de Valparaíso
Journal Title: Electronic Journal of Biotechnology
Volume: 13
Issue: 2
Publisher DOI: 10.2225/vol13-issue2-fulltext-7
Abstract: Despite the recent progress of transient gene expression systems in a red alga Porphyra yezoensis by particle bombardment, a stable transformation system has yet to establish in any marine red macrophytes. One of the reasons of the difficulty in genetic transformation in red algae is the lack of systems to select and isolate transformed cells from gametophytic blades. Thus, toward the establishment of the stable transformation system in P. yezoensis, we have developed a procedure by which transiently transformed gametophytic cells were prepared from particle bombarded-gametophytic blade as regeneratable protoplasts. Using mixture of marine bacterial enzymes, yield of protoplasts was high as reported elsewhere; however, these protoplasts did not develop. In contrast, protoplasts prepared from gametophytes treated with allantoin were normally developed, in which the overexpression of a β-glucuronidase reporter gene had no effect on the regeneration of protoplasts. Therefore, the use of allantoin in protoplast preparation sheds a new light on the realization of an efficient isolation and selection of study transformed cells from gametophytic blades.
Type: article (author version)
URI: http://hdl.handle.net/2115/44121
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

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