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Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis

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タイトル: Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis
著者: Takahashi, Megumu 著作を一覧する
Uji, Toshiki 著作を一覧する
Saga, Naotsune 著作を一覧する
Mikami, Koji 著作を一覧する
キーワード: allantoin
protoplast
P. yezoensis
regeneration
transient gene expression
発行日: 2010年 3月15日
出版者: Pontificia Universidad Católica de Valparaíso
誌名: Electronic Journal of Biotechnology
巻: 13
号: 2
出版社 DOI: 10.2225/vol13-issue2-fulltext-7
抄録: Despite the recent progress of transient gene expression systems in a red alga Porphyra yezoensis by particle bombardment, a stable transformation system has yet to establish in any marine red macrophytes. One of the reasons of the difficulty in genetic transformation in red algae is the lack of systems to select and isolate transformed cells from gametophytic blades. Thus, toward the establishment of the stable transformation system in P. yezoensis, we have developed a procedure by which transiently transformed gametophytic cells were prepared from particle bombarded-gametophytic blade as regeneratable protoplasts. Using mixture of marine bacterial enzymes, yield of protoplasts was high as reported elsewhere; however, these protoplasts did not develop. In contrast, protoplasts prepared from gametophytes treated with allantoin were normally developed, in which the overexpression of a β-glucuronidase reporter gene had no effect on the regeneration of protoplasts. Therefore, the use of allantoin in protoplast preparation sheds a new light on the realization of an efficient isolation and selection of study transformed cells from gametophytic blades.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/44121
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 三上 浩司

 

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