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Reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant Babesia gibsoni variants under low concentrations of diminazene aceturate

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.58.3-4.155

Title: Reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant Babesia gibsoni variants under low concentrations of diminazene aceturate
Authors: Hwang, Shiang-Jyi Browse this author
Yamasaki, Masahiro Browse this author →KAKEN DB
Nakamura, Kensuke Browse this author
Sasaki, Noboru Browse this author
Murakami, Masahiro Browse this author
Rajapakshage, Bandula Kumara Wickramasekara Browse this author
Ohta, Hiroshi Browse this author →KAKEN DB
Maede, Yoshimitsu Browse this author →KAKEN DB
Takiguchi, Mitsuyoshi Browse this author →KAKEN DB
Keywords: Babesia gibsoni
drug resistance
heat shock protein 70
qRT-PCR
selection
Issue Date: Nov-2010
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 58
Issue: 3&4
Start Page: 155
End Page: 164
Abstract: In our previous report, we developed a diminazene aceturate (DA)-resistant Babesia gibsoni strain that was maintained in culture with 200 ng/ml DA. While developing this strain, we also obtained DA-resistant B. gibsoni variants, which were maintained in culture with DA from 1 to 175 ng/ml for more than 8 weeks. Because heat shock protein 70 (Hsp70) seems to play important roles in adaptation to a stress environment in protozoan parasites, in the present study, we examined the copy number of B. gibsoni Hsp70 (BgHsp70) transcripts of those DA-resistant variants using quantitative real-time reverse transcription-polymerase chain reaction. We found that when wild-type B. gibsoni was exposed to 1 ng/ml DA, the level of BgHsp70 transcripts was decreased at day 14. The copy number of BgHsp70 transcripts in the DA-resistant variant cultured with 1 ng/ml DA was significantly lower than in wild-type B. gibsoni, while those in DA-resistant variants increased with escalating doses of DA from 1 to 75 ng/ml, although they were lower than in wild-type B. gibsoni. However, those in DA-resistant variants cultured with > 125 ng/ml DA were almost the same as wild-type B. gibsoni. These results indicated that the transcript levels of the BgHsp70 gene might be reduced when the parasites are exposed to a low concentration of DA, and then might recover to the normal level after achieving resistance against DA. We expect that further study of the function of BgHsp70 will elucidate the mechanism of drug resistance against DA in B. gibsoni.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/44137
Appears in Collections:Japanese Journal of Veterinary Research > Volume 58, Number 3&4

Submitter: 獣医学部図書室

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