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Measurement of lipoprotein particle sizes using dynamic light scattering

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タイトル: Measurement of lipoprotein particle sizes using dynamic light scattering
著者: Sakurai, Toshihiro 著作を一覧する
Trirongjitmoah, Suchin 著作を一覧する
Nishibata, Yuka 著作を一覧する
Namita, Takeshi 著作を一覧する
Tsuji, Masahiro 著作を一覧する
Hui, Shu-Ping 著作を一覧する
Jin, Shigeki 著作を一覧する
Shimizu, Koichi 著作を一覧する
Chiba, Hitoshi 著作を一覧する
発行日: 2010年 9月
出版者: Royal Society of Medicine Press
誌名: Annals of Clinical Biochemistry
巻: 47
号: 5
開始ページ: 476
終了ページ: 481
出版社 DOI: 10.1258/acb.2010.010100
抄録: Background: A simple method for the measurement of LDL particle sizes is needed in clinical laboratories because a predominance of small dense LDL (sd LDL) has been associated with coronary heart disease. We applied dynamic light scattering (DLS) to measure lipoprotein particle sizes, with special reference to sd LDL. Methods: Human serum lipoproteins isolated by a combination of ultracentrifugation and gel chromatography, or by sequential ultracentrifugation, were measured for particle size using DLS. Results: The sizes of polystyrene beads, with diameters of 21 and 28 nm according to the manufacturer, were determined by DLS as 19.3 ± 1.0 nm (mean ± SD, n = 11) and 25.5 ± 1.0 nm, respectively. The coefficients of variation for the 21-nm and 28-nm beads were 5.1% and 3.8% (within-run, n = 11), and 2.9% and 6.2% (between-run, n = 3), respectively. The lipoprotein sizes determined by DLS for lipoprotein fractions isolated by chromatography were consistent with the elution profile. Whole serum, four isolated lipoprotein fractions (CM + VLDL + IDL, large LDL, sd LDL, and HDL) and a non-lipoprotein fraction isolated by sequential ultracentrifugation were determined by DLS to be 13.1 ± 7.5 nm, 37.0 ± 5.2 nm, 21.5 ± 0.8 nm, 20.3 ± 1.1 nm, 8.6 ± 1.5 nm, and 8.8 ± 2.0 nm, respectively. Conclusions: The proposed DLS method can differentiate the sizes of isolated lipoprotein particles, including large LDL and sd LDL, and might be used in clinical laboratories in combination with convenient lipoprotein separation.
Rights: Ann Clin Biochem 2010;47:476-481, doi:10.1258/acb.2010.010100. This is the final draft, after peer-review, of a manuscript published in Annals of Clinical Biochemistry. The definitive version, detailed above, is available online at www.rsmjournals.com.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/44222
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 櫻井 俊宏

 

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