HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >

Measurement of lipoprotein particle sizes using dynamic light scattering

Files in This Item:
ACB47-5_476-481.pdf453.07 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/44222

Title: Measurement of lipoprotein particle sizes using dynamic light scattering
Authors: Sakurai, Toshihiro Browse this author
Trirongjitmoah, Suchin Browse this author
Nishibata, Yuka Browse this author
Namita, Takeshi Browse this author
Tsuji, Masahiro Browse this author
Hui, Shu-Ping Browse this author →KAKEN DB
Jin, Shigeki Browse this author
Shimizu, Koichi Browse this author →KAKEN DB
Chiba, Hitoshi Browse this author →KAKEN DB
Issue Date: Sep-2010
Publisher: Royal Society of Medicine Press
Journal Title: Annals of Clinical Biochemistry
Volume: 47
Issue: 5
Start Page: 476
End Page: 481
Publisher DOI: 10.1258/acb.2010.010100
PMID: 20736248
Abstract: Background: A simple method for the measurement of LDL particle sizes is needed in clinical laboratories because a predominance of small dense LDL (sd LDL) has been associated with coronary heart disease. We applied dynamic light scattering (DLS) to measure lipoprotein particle sizes, with special reference to sd LDL. Methods: Human serum lipoproteins isolated by a combination of ultracentrifugation and gel chromatography, or by sequential ultracentrifugation, were measured for particle size using DLS. Results: The sizes of polystyrene beads, with diameters of 21 and 28 nm according to the manufacturer, were determined by DLS as 19.3 ± 1.0 nm (mean ± SD, n = 11) and 25.5 ± 1.0 nm, respectively. The coefficients of variation for the 21-nm and 28-nm beads were 5.1% and 3.8% (within-run, n = 11), and 2.9% and 6.2% (between-run, n = 3), respectively. The lipoprotein sizes determined by DLS for lipoprotein fractions isolated by chromatography were consistent with the elution profile. Whole serum, four isolated lipoprotein fractions (CM + VLDL + IDL, large LDL, sd LDL, and HDL) and a non-lipoprotein fraction isolated by sequential ultracentrifugation were determined by DLS to be 13.1 ± 7.5 nm, 37.0 ± 5.2 nm, 21.5 ± 0.8 nm, 20.3 ± 1.1 nm, 8.6 ± 1.5 nm, and 8.8 ± 2.0 nm, respectively. Conclusions: The proposed DLS method can differentiate the sizes of isolated lipoprotein particles, including large LDL and sd LDL, and might be used in clinical laboratories in combination with convenient lipoprotein separation.
Rights: Ann Clin Biochem 2010;47:476-481, doi:10.1258/acb.2010.010100. This is the final draft, after peer-review, of a manuscript published in Annals of Clinical Biochemistry. The definitive version, detailed above, is available online at www.rsmjournals.com.
Type: article (author version)
URI: http://hdl.handle.net/2115/44222
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 櫻井 俊宏

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University