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Measurement of lipoprotein particle sizes using dynamic light scattering
Title: | Measurement of lipoprotein particle sizes using dynamic light scattering |
Authors: | Sakurai, Toshihiro Browse this author | Trirongjitmoah, Suchin Browse this author | Nishibata, Yuka Browse this author | Namita, Takeshi Browse this author | Tsuji, Masahiro Browse this author | Hui, Shu-Ping Browse this author →KAKEN DB | Jin, Shigeki Browse this author | Shimizu, Koichi Browse this author →KAKEN DB | Chiba, Hitoshi Browse this author →KAKEN DB |
Issue Date: | Sep-2010 |
Publisher: | Royal Society of Medicine Press |
Journal Title: | Annals of Clinical Biochemistry |
Volume: | 47 |
Issue: | 5 |
Start Page: | 476 |
End Page: | 481 |
Publisher DOI: | 10.1258/acb.2010.010100 |
PMID: | 20736248 |
Abstract: | Background: A simple method for the measurement of LDL particle sizes is needed in clinical laboratories because a predominance of small dense LDL (sd LDL) has been associated with coronary heart disease. We applied dynamic light scattering (DLS) to measure lipoprotein particle sizes, with special reference to sd LDL. Methods: Human serum lipoproteins isolated by a combination of ultracentrifugation and gel chromatography, or by sequential ultracentrifugation, were measured for particle size using DLS. Results: The sizes of polystyrene beads, with diameters of 21 and 28 nm according to the manufacturer, were determined by DLS as 19.3 ± 1.0 nm (mean ± SD, n = 11) and 25.5 ± 1.0 nm, respectively. The coefficients of variation for the 21-nm and 28-nm beads were 5.1% and 3.8% (within-run, n = 11), and 2.9% and 6.2% (between-run, n = 3), respectively. The lipoprotein sizes determined by DLS for lipoprotein fractions isolated by chromatography were consistent with the elution profile. Whole serum, four isolated lipoprotein fractions (CM + VLDL + IDL, large LDL, sd LDL, and HDL) and a non-lipoprotein fraction isolated by sequential ultracentrifugation were determined by DLS to be 13.1 ± 7.5 nm, 37.0 ± 5.2 nm, 21.5 ± 0.8 nm, 20.3 ± 1.1 nm, 8.6 ± 1.5 nm, and 8.8 ± 2.0 nm, respectively. Conclusions: The proposed DLS method can differentiate the sizes of isolated lipoprotein particles, including large LDL and sd LDL, and might be used in clinical laboratories in combination with convenient lipoprotein separation. |
Rights: | Ann Clin Biochem 2010;47:476-481, doi:10.1258/acb.2010.010100.
This is the final draft, after peer-review, of a manuscript published in Annals of Clinical Biochemistry. The definitive version, detailed above, is available online at www.rsmjournals.com. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/44222 |
Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 櫻井 俊宏
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