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Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus

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Title: Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus
Authors: Li, Ya-Juan Browse this author
Tian, Yi Browse this author
Zhang, Ming-Zhao Browse this author
Tian, Ping-Ping Browse this author
Yu, Zhou Browse this author
Abe, Syuiti Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: Ag-NORs
Cobitidae
Fluorescence in-situ hybridization
Polyploidy
Ribosomal genes
Weatherfish
Issue Date: 2010
Publisher: Springer
Journal Title: Ichthyological Research
Volume: 57
Issue: 4
Start Page: 358
End Page: 366
Publisher DOI: 10.1007/s10228-010-0168-0
Abstract: The chromosomes of diploid and tetraploid loach, Misgurnus anguillicaudatus, were analyzed by staining with Ag, Chromomycin A3 (CMA3)/ Distamycin A (DA), and DA/4',6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizing regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in diploid loach with 2n=50 and the homologous quartet in tetraploid loach with 4n=100. The NORs were positive at the same region of the first metacentric chromosome for Ag- and CMA3/DA-stainings, but negative for DA/DAPI-staining. Four signals at the homologues within the same quartet suggest the duplication of entire genome from diploid to tetraploid status. However, size difference was detected among rDNA signals by FISH and CMA3-banding.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/44223
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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