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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus
| Title: | Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus |
| Authors: | Li, Ya-Juan Browse this author | | Tian, Yi Browse this author | | Zhang, Ming-Zhao Browse this author | | Tian, Ping-Ping Browse this author | | Yu, Zhou Browse this author | | Abe, Syuiti Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | Ag-NORs | | Cobitidae | | Fluorescence in-situ hybridization | | Polyploidy | | Ribosomal genes | | Weatherfish |
| Issue Date: | 2010 |
| Publisher: | Springer |
| Journal Title: | Ichthyological Research |
| Volume: | 57 |
| Issue: | 4 |
| Start Page: | 358 |
| End Page: | 366 |
| Publisher DOI: | 10.1007/s10228-010-0168-0 |
| Abstract: | The chromosomes of diploid and tetraploid loach, Misgurnus anguillicaudatus, were analyzed by staining with Ag, Chromomycin A3 (CMA3)/ Distamycin A (DA), and DA/4',6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizing regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in diploid loach with 2n=50 and the homologous quartet in tetraploid loach with 4n=100. The NORs were positive at the same region of the first metacentric chromosome for Ag- and CMA3/DA-stainings, but negative for DA/DAPI-staining. Four signals at the homologues within the same quartet suggest the duplication of entire genome from diploid to tetraploid status. However, size difference was detected among rDNA signals by FISH and CMA3-banding. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44223 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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